流式免疫多重微球芯片技术检测干燥综合征自身抗体的评价及应用  

Establishment and clinical application of multiplex flow cytometric immuno-bead array assay technology in detecting primary sjogren′s syndrome

作　　者：牛广华[1] 高玉洁[1] 张程[1] 张艺凡[1] 吕丹[1] 崔百慧 

机构地区：[1]辽宁中医药大学附属医院,沈阳110032

出　　处：《检验医学与临床》2016年第14期1911-1913,共3页Laboratory Medicine and Clinic

基　　金：辽宁省自然基金课题(2014010058-301)

摘　　要：目的建立多重微球芯片流式免疫荧光发光法检测原发性干燥综合征(pSS)自身抗体的评价及应用。方法采用多重微球流式免疫荧光发光法,以聚苯乙烯微球为反应界面,将8种[SSA(60kD)、SSB、α-fodrin、M3、ds-DNA、nRNP/Sm、Sm、M2]抗人IgG单克隆抗体包被微球后,用该方法检测同一血清样本中的多种可溶性蛋白抗体。选取pSS患者206例、非pSS患者110例和健康人69例,用多重微球流式免疫荧光发光法检测8种自身抗体。结果多重微珠流式免疫荧光发光法检测8种抗体的批内差异及批间差异均较小;检测pSS组的8种抗体水平与非pSS组、健康组比较,差异均有统计学意义(P<0.01);诊断pSS的总敏感度可达97.20%,总特异性可达96.34%。结论多重微球流式免疫荧光发光法检测pSS自身抗体方法可以一次同时进行8种自身抗体的联合检测,为pSS及其他自身免疫病的确诊创造了一种准确、便捷的检测新方法。Objective To establish and applicata a method of multiplex flow cytometric immuno‐bead array assay technology in detecting primary sjogren′s syndrome （pSS） .Methods By multiplex flow cytometric immuno‐bead array assay technology ,polysty‐rene microspheres were coated by eight monoclonal antibodies [SSA （60 kD ） ,SSB ,α‐fodrin ,M3 ,ds‐DNA ,nRNP/Sm ,Sm ,M2 ] , which were also regarded as reaction interface .And the varieties of soluble protein were detected in one sample at the same time by this kind of method .A total of 206 pSS patients ,110 non‐pSS patients and 96 healthy people were selected as subjects of our study . The new method was used for detecting 8 autoantibodies in the pSS group ,non‐pSS group and normal group .Results The coeffi‐cient of variation （CV） of intra‐repetition and inter‐repetition were small in difference in detection of 8 autoantibodies ;compared with the pSS group ,the difference in the non‐pSS group and normal group had statistical significance（P〈0 .01） .The sensitivity for the diagnosis of pSS group was 97 .20% and specificity was 96 .34% .Conclusion Multiplex flow cytometric immuno‐bead array as‐say technology can be used to detect eight antibodies of pSS ,which might provide for clinical diagnosis of pSS and other autoimmune diseases a quick and accurate method .

关 键 词：原发性干燥综合征 多重微球芯片流式免疫荧光发光法 血清生物标志物 自身抗原 自身抗体 

分 类 号：R442.8[医药卫生—诊断学]