基于EST-SSR分子标记的青海高原以礼草属主要物种的遗传多样性分析  

Genetic Diversity of the Major Varieties of Kengyilia C. Yen et J. L.Yang in Qing Hai Plateau Based on EST-SSR Markers

作　　者：任建东[1] 李凤珍[2] 许媛君 王晓醒 马晓岗[1] 

机构地区：[1]青海省农林科学院,西宁810016 [2]青海大学环境工程学院,西宁810016 [3]青海大学农牧学院,西宁810016

出　　处：《植物遗传资源学报》2016年第4期663-670,共8页Journal of Plant Genetic Resources

基　　金：青海省干旱区抗逆农作物种质资源调查(2011FY110200-7);青海高原特色冷凉作物优异种质创新与利用(2013BAD01B05-3);青海小麦近缘种以礼草属遗传多样性研究(2012-QNY-9)

摘　　要：利用EST-SSR分子标记技术对青海高原以礼草属3个物种的21份材料进行遗传相关分析。遗传多样性分析发现97对引物中有50对引物可扩增出特异性条带,共扩增出103条条带,各个引物的扩增条带数范围为1~5,每个位点的平均变异数2.06。Popgene pc软件分析表明：梭罗草遗传多样性水平最高（PP=62.14%,h=0.2345）,糙毛以礼草次之（PP=58.25%,h=0.2211）,大颖草最低（PP=44.66%,h=0.1687）;梭罗草和大颖草的遗传距离最小,遗传相似性程度最高,糙毛以礼草与梭罗草和大颖草的遗传距离均较远。梭罗草居群中玛多梭罗草与其他居群遗传距离相对较远,糙毛以礼草居群中克图尼哈糙毛以礼草与其他居群遗传距离相对较远,大颖草居群中克图尼哈大颖草与其他居群遗传距离相对较远。NTSYS2.10e聚类结果为21个居群可聚为3个组群,梭罗草组群10个;糙毛以礼草组群7个;大颖草组群4个。本研究可进一步为拓宽小麦族植物的遗传基础、野生基因资源利用及研究物种分布规律提供科学参考。The genetic diversity of 21 populations collected from 3 varieties of Kengyilia C. Yen et J. L. Yang was investigated by EST-SSR markers. There were 103 polymorphic bands produced by 60 out of 97 EST-SSR primers in those materials. Every primers amplified 1 to 5 bands,and every band had an average number of 2. 06. The genetic diversity of Kengyilia thoroldiana was the highest followed by Kengyilia hirsuta,while Kengyilia grandiglumis showed the lowest PP of 44. 66% in genetic diversity. Genetic similarity analysis suggested that the distance between K. thoroldiana and K. grandiglumis was minimal,i. e.,the genetic similarity was maximum. The genetic distance was far between K. hirsuta and K. grandiglumis and Kengyilia thoroldiana. The genetic distance was approximately 0. 345 comparing Maduo＇s population of K. thoroldiana with other populations. Similarly,genetic distance between Ketunika＇s population of K. hirsuta comparing with other populations was still significant. The 21 populations was classified 3groups when the genetic similarity coefficient was about 0. 65,including 10 K. thoroldiana populations in group 1,7 K.hirsuta populations in group 2 and 4 K. grandiglumis populations in group 3. The results of this study should providevaluable information and reference for future development and protection of wild gene resources in Triticeae.

关 键 词：以礼草属 EST-SSR分子标记 遗传多样性 聚类分析 

分 类 号：Q943[生物学—植物学]