大麦HvLEC1基因的克隆及其表达特征分析  

Cloning and Expression Characterization of HvLEC1 in Hordeum vulgare L.

作　　者：李颖波[1] 郭桂梅[1] 刘成洪[1] 何婷[1] 高润红[1] 徐红卫[1] 陈志伟[1] 陆瑞菊[1] 黄剑华[1] 

机构地区：[1]上海市农业科学院生物技术研究所/遗传育种重点实验室,上海201106

出　　处：《植物遗传资源学报》2016年第4期732-737,共6页Journal of Plant Genetic Resources

基　　金：上海市自然科学基金项目(15ZR1436600);大麦青稞产业技术体系(CARS-05);上海市种业发展项目[(2015)第3号]

摘　　要：植物LEC蛋白是NF-Y转录因子的一类B亚基,在植物胚状体形成过程中起重要作用。为了研究大麦小孢子体外培养形成胚状体的机理,本研究利用RACE技术在大麦中克隆了一个新的LEC基因,该基因cDNA全长为1004 bp,开放阅读框全长为597 bp,编码198个氨基酸,其蛋白1~59位氨基酸含有LEC结构域,命名为HvLEC1。HvLEC1在大麦的根、茎、叶和小孢子培养过程中均能表达,其中小孢子培养7 d时表达量最高,且HvLEC1在大麦品系BI04中的表达量比基19高,BI04愈伤产量也比基19高,表明HvLEC1表达量和愈伤产量有相关性,受盐胁迫后HvLEC1在大麦的根中快速上调表达,提示HvLEC1可能不仅参与小孢子胚状体发生,而且参与盐胁迫响应。Plant LEAFY COTYLEDON（ LEC） proteins,which belong to the NF-Y transcription factor B subunit,play central role in plant embryogenesis. To investigate the mechanism of microspore induced embryo in vitro in barley,a novel LEC gene,Hv LEC1,was cloned from barley using the RACE method. The isolated full-length c DNA sequence of Hv LEC1 was 1004 bp,containing a 597 bp open reading frame（ ORF） coding for 198 amino acids. The deduced amino acid sequence of Hv LEC1 contained a LEC domain located between amino acids 1 and 59. Hv LEC1 can be detected in roots,stems,leaves,and the cultured microspores,and presented the highest expression in 7 days of cultured microspores. The expression of Hv LEC1 at 7 days of cultured microspores was higher in barley line BI04 than that in barley line Ji19,and callus yield at 21 days of cultured microspores was also higher in BI04 than that in Ji19,which revealed that the expression level of Hv LEC1 might be in consistent with the callus yield. The expression of Hv LEC1 can also be quickly up-regulated in barley root by salt stress,which suggested that Hv LEC1 may be involved in not only microspore embryogenesis but also the responses to salinity stress.

关 键 词：大麦 转录因子 小孢子 胚状体发生 盐胁迫 表达 

分 类 号：S512.3[农业科学—作物学]