肾系康方及其拆方亚组对系膜增生性肾炎模型大鼠肾组织α-SMA、HGF的影响  

Effect of Shenxikang on Renal Tissue α-SMA and HGF of Mesangial Proliferative Glomerulonephritis Model Rats

作　　者：吴文先[1] 高雅[1] 刘霞[2] 

机构地区：[1]河南省中医药研究院附属医院,河南郑州450004 [2]河南中医药大学第一附属医院,河南郑州450000

出　　处：《中医学报》2016年第7期1001-1003,共3页Acta Chinese Medicine

基　　金：河南省教育科技攻关计划项目(20013600009)

摘　　要：目的:观察肾系康全方及其益气活血组、滋阴清热组2个拆方组对系膜增生性肾小球肾炎(mesangial proliferative glomerulonephritis,Ms PGN)大鼠肾组织α平滑肌肌动蛋白(Alpha smooth muscle actin,α-SMA)、肝细胞生长因子(hepatocyte growth factor,HGF)的影响,以明确该方及2个组成部分发挥的作用强弱,并探讨其部分作用机理。方法:以雄性SD大鼠为研究对象,采用隔日口服牛血清白蛋白并尾静脉注射葡萄球菌肠毒素B及皮下分点注射完全氟氏佐剂和不完全氟氏佐剂的方法复制Ms PGN模型,应用免疫组化法检测各组大鼠肾组织中α-SMA、HGF表达水平并进行半定量分析。结果:肾系康方与2个拆方组均可降低肾组织中α-SMA表达,上调肾组织中HGF表达,与模型对照组比较,差异有统计学意义(P<0.05);拆方组中,益气活血组明显优于滋阴清热组,两组比较,差异有统计学意义(P<0.05);各治疗组作用强度为全方组>益气活血组>滋阴清热组。结论:肾系康方能够抑制肾组织α-SMA的表达,上调HGF的表达,可能是其防治肾小球硬化和肾脏纤维化的作用机制之一;肾系康方及其拆方均有明显作用,益气活血组明显优于滋阴清热组,而全方组更为显著。Objective： To observe the effect of Shenxikang on mesangial proliferative glomerulonephritis（ Ms PGN）,Alpha smooth muscle actin（ α-SMA） and hepatocyte growth factor（ HGF） and to illustrate the mechanism of the process. Methods： In this study,male SD rats were used as the research objects. Every other day with oral use of bovine serum albumin and intravenous injection of staphylococcal enterotoxin B and by complete subcutaneous points injection and incomplete subcutaneous point injection Ms PGN model rats were made. Immunohistochemical method was used to detect the renal tissue α-SMA,HGF expression of rats and semiquantitative analysis was made. Results： The renal tissue level of α-SMA expression decreased and HGF expression significantly increased in Shenxikang group and other two related groups. Compared with those of the control group,the differences were statistically significant（ P 〈0. 05）. The improvement of those indexes of the Ziyinqingre group were significantly better than that of the Yiqihuoxue group（ P 〈0. 05）. The effective order of each treatment group was Shenxikang group Yiqihuoxue group Ziyinqingre group. Conclusion： Shenxikang can achieve significant medical effect in inhibit the expression of α-SMA in renal tissue and increase the level of HGF. One of the mechanisms may be the prevention of glomerulosclerosis and renal fibrosis.

关 键 词：肾系康方 Α平滑肌肌动蛋白 肝细胞生长因子 

分 类 号：R285.5[医药卫生—中药学]