冬凌草有效部位中总黄酮含量测定  

Determination of Total Flavonoids Content in Effective Part of Rabdosia Rubescens

作　　者：王灿[1] 方晓艳[2] 苗明三[2] 

机构地区：[1]天津中医药大学,天津300193 [2]河南中医药大学,河南郑州450046

出　　处：《中医学报》2016年第7期1018-1020,1035,共4页Acta Chinese Medicine

基　　金：国家自然科学基金项目(U1204827);河南省高校科技创新团队项目(2012IRTSTHN011);郑州市科技创新团队项目(131PCXTD612)

摘　　要：目的:建立冬凌草有效部位中总黄酮含量测定的方法。方法:以芦丁为对照品,采用氢氧化钠、亚硝酸钠和硝酸铝作为显色剂,在510 nm波长下测定冬凌草有效部位中总黄酮的含量。结果:芦丁的线性范围为0.021~0.105 g·L^(-1),r=0.999 2,平均加样回收率为101.0%(RSD=1.86%,n=9)。结论:所建立的含量测定方法简便、快速,稳定性和重复性良好,适用于测定冬凌草有效部位中总黄酮的含量。Objective： To establish the method of the content determination of total flavonoids in the effective part of Rabdosia rubescens. Method： Ultraviolet spectrophotometry was applied by using aluminum nitrate,sodium nitrite and Na OH as colour-display reagent and using rutin as reference substance. The detection wavelength was set at 510 nm. Result： The linear range of rutin was 0. 021-0. 105 mg·m L^（-1）（ r = 0. 999 2） with an average recovery of 101. 0%（ RSD = 1. 86%,n = 9）. Conclusion： the method is accurate,simple,rapid with good reproducibility,which is suitable for the content determination of total flavonoids in the effective part of Rabdosia rubescens.

关 键 词：冬凌草 总黄酮 紫外分光光度法 含量测定 

分 类 号：R284.2[医药卫生—中药学]