黄连阿胶汤不同煎煮方法比较  

Use HPLC Method to Establish Characteristic Spectrum and Comparative Studies on Different Decoction Boiling Methods of Huanglian Ejiao Tang

作　　者：邓德英[1] 贾利利 张振凌[1] 

机构地区：[1]河南中医药大学,呼吸疾病诊疗与新药研发河南省协同创新中心,河南郑州450046 [2]佐今明制药股份有限公司,河南新乡453700

出　　处：《中医学报》2016年第7期1021-1025,共5页Acta Chinese Medicine

基　　金：国家中医药管理局行业科研专项(201007010)

摘　　要：目的:建立黄连阿胶汤高效液相特征图谱,比较不同煎煮方法在特征图谱上化学成分的差异。方法:采用高效液相色谱法,建立黄连阿胶汤的特征图谱,并分析不同煎煮方法的黄连阿胶汤样品出峰位置、数量及峰面积上的差别。结果:采用高效液相色谱法建立了黄连阿胶汤的特征图谱,利用指纹图谱处理软件Chromap1.5对该图谱进行相似度分析,得出9批黄连阿胶汤特征图谱相似度不低于0.9,同时通过图谱处理软件比较了不同煎煮方法的黄连阿胶汤样品,样品之间特征谱差别不大。结论:可以采用指纹图谱控制汤剂煎煮质量;黄连阿胶汤传统煎煮法、机械常压煎煮法、机械高压煎煮法均可达到汤剂煎煮的疗效和质量要求。Objective： To Establish Huanglian Ejiao Tang characteristic spectrum and to compare different decocting methods of the differences in chemical composition. Methods： Use HPLC method to establish the characteristic spectrum of Huanglian Ejiao Tang and analyze different boiling methods of Huanglian Ejiao Tang in the peak position,quantity and peak area. Results： By HPLC method to establish the characteristic spectrum of Huanglian Ejiao Tang,by the fingerprint processing software Chromap1. 5 the characteristic spectrum similarity analysis,the spectrum similarity of nine batches Huanglian Ejiao Tang characteristic was not less than 0. 9,compared different boiling method Huanglian Ejiao Tang characteristic spectrum was similar. Conclusion： By comparing characteristics spectral of Huanglian Ejiao Tang samples of boiling traditional,mechanical normal pressure and mechanical high pressure,no significant difference between the three samples was found.

关 键 词：黄连阿胶汤 煎煮方法 高效液相色谱法 特征图谱 

分 类 号：R284.2[医药卫生—中药学]