大豆总皂甙对运动力竭大鼠肾脏自由基代谢的影响  

The Effects of Total Soy Saponins on Free Radicals in the Kidney of Exhausted Rats

作　　者：刘志刚[1] 熊正英[2] 刘琴[3] 张朝会[3] 

机构地区：[1]玉溪师范学院体育学院,云南玉溪653100 [2]陕西师范大学体育学院,陕西西安710062 [3]玉溪师范学院资源与环境学院,云南玉溪653100

出　　处：《云南师范大学学报（自然科学版）》2016年第4期62-65,共4页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家自然科学基金资助项目(51568066)

摘　　要：SD大鼠随机分为四个组:对照组、安静皂甙组、力竭组和力竭皂甙组.安静皂甙组和力竭皂甙组按照20mg/kg经口腔给药,每日一次,持续2周,力竭组和对照组灌服相同体积生理盐水.2周后进行一次跑台急性力竭运动.动物力竭后即刻取材,分离肾脏,制成10%组织匀浆.分光光度法测试肾组织SOD、CAT、MDA、GSH-Px、GR、GSH和T-AOC;各组数据进行单因素方差分析、S-N-K检验并计算效应量Cohen′s d值.结果显示:(1)TS能显著延长大鼠力竭运动时间;(2)相比于对照组,安静皂甙组SOD和GR活性显著升高(P<0.05),而MDA显著降低(P<0.01);相比于力竭组,力竭皂甙组SOD活性显著升高(P<0.05);相比于安静皂甙组,力竭皂甙组GR活性降低(P<0.05)而MDA水平显著升高(P<0.05);(3)TS对运动力竭大鼠肾脏各抗氧化指标显示出了正性的影响,尤其是提升SOD活性.All rats were divided into four groups：control group,TS group, exhausted group and TS exhausted group. TS group and TS exhausted group were fed with TS through the mouth with a dosage of 20 mg/kg body weight, once a day for two weeks. The exhausted group and control group were given placebo. Treadmill speed was set at 30 m/min,and the rats（exhausted group and TS exhausted group） were trained at this speed until exhausted. Then the rats were decapitated and anatomized immediately. 10% homogenate of myocardial tissue was prepared. The results show that（1）TS can significantly increase the rats exercise time by 20. 62% （P〈0.05）; （2）Compared with the control group,the enzyme activity of SOD and GR significantly enhanced in TS group（ P 〈0. 05）;MDA level significantly decreased in TS group（ P〈0. 01）;Compared with the exhausted group, MDA level significantly decreased in TS exhausted group（ P〈0.05）; Compared with TS group,the GR activity significantly decreased（ P〈0. 05） and MDA level increased in TS exhausted group（ P〈0. 05）;（3）TS has a positive effect on the antioxidase activity of kidney in exercised rats,especially increase SOD activity.

关 键 词：大豆总皂甙 抗氧化 力竭运动大鼠 肾脏 

分 类 号：G804.2[文化科学—运动人体科学] Q946.83[文化科学—体育学]