不同世代间大豆粒形相关QTL的稳定性分析  

Stability Analysis of QTL Associated for Seed Shape Traits in Soybean Content Across Different Generations

作　　者：张继雨 郭洁[1] 陈峰娜 李文[1] 赵雪[1] 韩英鹏[1] 李文滨[1] 滕卫丽[1] 

机构地区：[1]东北农业大学大豆研究所/大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室,黑龙江哈尔滨150030

出　　处：《大豆科学》2016年第4期581-586,共6页Soybean Science

基　　金：国家自然科学基金面上项目(31471517);黑龙江省教育厅科技项目(10531012);国家现代农业产业技术体系(CARS-04-PS04);国家"十二五"科技支撑计划(2011BAD35B06;2013BAD20B04)

摘　　要：以东农46和L-100构建的重组自交系群体（RILs）为试验材料,利用2013和2014年分别在哈尔滨、呼兰、阿城3个地点共6个环境的数据对不同世代的遗传群体粒形进行单环境QTL分析及多环境联合检测。结果表明：单环境QTL分析中,检测到13个与粒形相关的QTL,位于第5、9、12、15及18连锁群上,其中粒长QTL 2个,表型变异贡献率为21.61%～26.81%;粒宽QTL 5个,表型变异贡献率为7.28%～18.38%;粒厚QTL 6个,表型变异贡献率为10.19%～18.44%。位于Sat_122～Satt052标记区间的QTL位点在粒长及粒厚中都被检测到,位于Sat_119～Satt588、Satt192～Satt568及Sat_401～Satt192标记区间QTL位点在粒宽及粒厚中同时被检测到,存在一因多效性。多环境联合分析中,共检测到15个与粒形相关的QTL。并且有9个QTL位点在单环境及多环境联合检测中均被检测到,表明这些QTL表达较为稳定。因此,本研究获得1个粒长QTL（Sat_122～Satt052）、1个粒宽QTL（Satt192～Satt568）及2个粒厚QTL（Satt192～Satt568,Sat_401～Satt192）为表现较好的稳定QTL。A RILs population derived from a cross between Dongnong 46 and L-100 was used in the experiment. The different generation populations were evaluated in three locations（ Harbin, Hulan and Acheng） , in 2013 and 2014 using single-enviroment QTL analysis and muhi-enviroment joint analysis. The results showed that, thirteen QTLs were detected associated with seed shape in single-enviroment QTL analysis, located in 5, 9, 12, 15, and 18 linkage group. Two QTLs were associated with seed length, explained 21.61% -26. 81% of the phenotypie variation. Five QTLs were associated with seed width, ex- plained 7. 28% - 18. 38% of the phenotypic variation. Six QTLs were associated with seed thick, explained 10. 19% - 18.44% of the phenotypic variation. The QTLs located in the marker interval of Sat_122 - Satt052 associated both seed length and seed thick. The QTLs located in the marker interval of Sat_119 - Satt588, Satt192 - Satt568 and Sat_401 - Satt192 asso- ciated both seed width and seed thick, showed pleiotropy. Fifteen QTLs were detected associated with seed shape in muhi-enviroment joint analysis. Nine QTLs were detected in both methods, showed genetic stability.

关 键 词：大豆 RILs 粒形 QTL 稳定遗传 

分 类 号：S565.1[农业科学—作物学]