摩西管柄囊霉与连作大豆根腐病原菌尖孢镰刀菌的相互关系研究  

Research on the Relationship Between Funneliformis mosseae and the Root Rot Pathogen Fusarium Oxysporum in the Continuous Cropping of Soybean

作　　者：接伟光[1] 于文杰[2] 蔡柏岩[2] 

机构地区：[1]黑龙江东方学院食品与环境工程学部,黑龙江哈尔滨150066 [2]黑龙江大学生命科学学院/黑龙江省普通高等学校微生物重点实验室,黑龙江哈尔滨150080

出　　处：《大豆科学》2016年第4期637-642,共6页Soybean Science

基　　金：黑龙江省普通高等学校青年学术骨干支持计划(1254G049)

摘　　要：以黑农44(高脂肪品种)大豆品种作为试验材料,以不同方式在连作大豆根际土壤中接种摩西管柄囊霉(Funneliformis mosseae)和大豆根腐病原菌尖孢镰刀菌(Fusarium oxysporum)。采用传统形态学方法检测Fu.mosseae和F.oxysporum侵染大豆植株根系情况。利用实时荧光定量PCR技术分析Fu.mosseae和F.oxysporum DNA含量变化。结果表明:Fu.mosseae和F.oxysporum均能侵染大豆植株根系,但接种Fu.mosseae的大豆植株根系及根际土壤样品中F.oxysporum DNA含量显著降低,表明Fu.mosseae对F.oxysporum具有强烈的抑制作用。本研究为进一步研究AM真菌克服大豆连作障碍提供了理论依据。In this study, Heinong 44 （a high-fat cuhivar） soybean cuhivar was selected as materials. Funneliformis mosseae and the root rot pathogen Fusarium oxysporum were inoculated into the soybean continuous cropping soil in different treatments. The colonisation rates of Fu. mosseae and disease incidences of F. oxysporum were analyzed statistically, respectively. Quantitative PCR was applied to analyze the DNA content of Fu. mosseae and F. oxysporum. The results showed that both Fu. mosseae and F. oxysporum could infect the soybean roots. However, the DNA content of F. oxysporum clearly decreased in soybean root and rhizosphere soil samples after the inoculation with Fu. mosseae. It might indicate inhibitive effects of Fu. mosseae over F. oxysporum. It provides theoretical evidence on the AM fungi to overcome the obstacles of soybean continuous cropping.

关 键 词：大豆 摩西管柄囊霉 尖孢镰刀菌 连作 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]