临床生物化学实验室用水管理探讨  

Evaluation on the Importance of Quality Control of Water Used for Clinical Chemistry Laboratory

作　　者：陈燕[1] 康熙雄[1] 孔凡虹 黄泽玉[1] 李宇[1] 陈兰枝[1] 刘竞争[1] 孙育[1] 钱芳[1] 李冉冉[1] 田硕[2] 尚大雷[3] 王雅杰[1,4] 

机构地区：[1]首都医科大学附属北京天坛医院检验科,北京100050 [2]首都医科大学附属北京儿童医院,北京100045 [3]北京市大兴区人民医院检验科,北京102600 [4]首都医科大学附属北京天坛医院临床医学研究实验室,北京100050

出　　处：《标记免疫分析与临床》2016年第7期834-837,共4页Labeled Immunoassays and Clinical Medicine

基　　金：首都医科大学校长基金(2015JYY80)

摘　　要：目的探讨临床生物化学实验室用水按微生物监测分级使用的重要性。方法水质微生物培养在一级标准和二级标准两种条件下,以二氧化碳浓缩试剂为测定试剂比较不同微生物培养结果时对校准工作曲线的影响;比较使用浓缩试剂和非浓缩试剂对测定二氧化碳的影响。用注射用水和实验室水机所制纯水分别复溶甘油三酯干粉试剂后,观察试剂颜色变化和试剂空白吸光度,比较不同实验室试剂用水等级对干粉试剂的影响。结果微生物培养结果为一级标准的纯水,可以用于试剂溶剂和仪器清洗,微生物培养结果为二级标准的纯水,不能作为试剂溶剂,只能用于设备清洗。注射用水可用于复溶甘油三酯干粉试剂,本实验纯水机制备的纯水不适用于复溶甘油三酯干粉试剂。结论建议医学实验室每天监控纯水机电阻率,每月对纯水机水箱进行微生物培养,不同等级纯水用于不同实验室操作。医学实验室用水定期检测(微生物含量、pH值范围等)对于实验室风险评估、保证实验室质量非常必要。Objective To evaluate the importance of grading water based on the microbiological culture results for clinical chemistry laboratory. Methods The water-quality was defined as primary standard, and secondary standard with microbial culture analysis. Concentrated carbon dioxide reagent was chosen for drawing the calibration curve in order to compare different microbiological culture results. Comparing the impact of non-concentrated reagents and concentrated reagents for the determination of carbon dioxide. Reconstitution of triglyceride powder with water-for-injection and the water produced by purifier. The color and calibration result of the solution was monitored. Results Pure water of primary standard tested by microbial culture can be used as equipment cleaning solvent and reagent solvent. The secondary standard pure water tested by microbial culture cannot be used as a reagent solvent. It can only be used to clean equipment. Water-for-injection can be used for reagent solvent. The water produced by purifier in our lab is not suitable as reagent solvent. Conclusion We recommend that medical laboratories should monitor the resistivity of water purifiers daily. Microbiological culture testing for sampling out of water purification tanks should be performed monthly. Different grades of water should be used in different experiments. It is essential to conduct regular checks of microbes, pH, and the conductivity of water applied in clinical laboratories.

关 键 词：质量控制 实验室用水 风险管理 临床生物化学实验室 

分 类 号：R446.1[医药卫生—诊断学]