激光照射足三里穴对慢性萎缩性胃炎大鼠胃酸分泌及NO含量的影响  

Effects of acupoint laser irradiation on gastric acid secretion and NO content in chronic atrophic gastritis

作　　者：邵雪辉[1] 王建国[4] 张晓丽[2] 高建武[1] 朱晓波[3] 杨跃平[1] 

机构地区：[1]河北北方学院医学物理教研室,张家口075000 [2]河北北方学院医学物理教研室电镜室,张家口075000 [3]河北北方学院生化教研室,张家口075000 [4]河北北方学院张家口学院,张家口075000

出　　处：《中华物理医学与康复杂志》2016年第7期492-496,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：河北省科技支撑项目（14277778D）;河北省卫生厅科研基金项目（20150051）

摘　　要：目的探讨氦氖激光照射足三里穴对慢性萎缩性胃炎（CAG）大鼠胃液pH值、胃黏膜壁细胞超微结构及血清中一氧化氮（NO）含量的影响。方法采用随机数字表法将60只健康雄性Wistar大鼠分成正常对照组、模型组、药物组、针刺组及激光穴位组。通过给予水杨酸钠、酒精灌胃并结合禁食禁水、跑步劳累等干预将模型组、药物组、针刺组及激光穴位组大鼠制成CAG动物模型。确认造模成功后，药物组、针刺组及激光穴位组大鼠分别给予药物（如延参健胃胶囊、康复新液灌注）、针刺、激光穴位照射干预。于治疗14d后断头取血，采集稀释胃液及部分胃组织；利用硝酸还原酶法检测血清中NO含量，采用酸度计测定胃液pH值，通过透射电镜观察胃黏膜超微结构变化。结果模型组大鼠胃液pH值、血清中NO含量均较正常对照组明显升高（P〈0．05），其壁细胞胞膜结构缺失、部分溶解，线粒体肿胀。药物、针刺足三里穴及激光照射足三里穴均能降低胃液pH值及血清中NO含量，并且以激光穴位组胃液pH值及血清中NO含量的改善情况尤为显著，与模型组间差异具有统计学意义（P〈0．05），其壁细胞胞膜结构完整，胞浆细胞器基本恢复正常。结论激光照射足三里穴能促进CAG大鼠壁细胞结构及功能恢复，提高胃酸分泌并降低血清NO水平，对缓解CAG病情具有较好疗效，该疗法值得临床进一步研究、推广。Objective To study the effects of He-Ne laser irradiation on the pH value of gastric juice, the uhrastructure of gastric parietal cells and the content of nitric oxide （NO） in chronic atrophic gastritis （ CAG）. Methods Sixty healthy male Wistar rats were randomly divided into a normal control group, a model group, a drug group, an acupuncture group and a laser acupoint group, each of 12. A CAG model was successfully intro- duced to all except those in the normal control group through the administration of sodium salicylate and alcohol, combined with fasting and water deprivation, running fatigue and other interventions. The rats of the drug, acupunc- ture and laser acupuncture groups were given medications, acupuncture at Zusanli acupoint and laser irradiation at Zusanli acupoint accordingly. After 14 days of treatment, all of the rats were executed and diluted gastric juice and gastric tissues were collected. NO content in serum was determined using a nitrate enzyme reduction method, the pH value of gastric juice was measured, and the ultrastrueture of the gastric nmcosa was observed using a transmis- sion electron microscope. Results The average pH of the gastric juice and the average serum NO content were both significantly higher in the model group than in the normal control group. In the model group the membrane structure of the parietal cells was absent or partly dissolved and the rnitochondria were swollen. Drugs, acupuncture and laser irradiating at the Zusanli acupoint all corresponded with reduced pH and NO content, but the most signifi- cant improvements were observed in the laser acupuncture group. In the laser acupuncture group the membrane structure of parietal cells was complete, and the cytoplasmic organelles had nearly returned to normal. Conclusions Laser irradiation can promote recovery from CAG, increasing the secretion of gastric acid and de-creasing the serum level of NO. Both have a good curative effect for CAG. Laser irradiation deserves further clinical research and promotion.

关 键 词：激光穴位照射 慢性萎缩性胃炎 一氧化氮 超微结构 

分 类 号：R245[医药卫生—针灸推拿学]