外源物质诱导对甜瓜枯萎病抗性和防御酶活性的影响  

Induction Resistance and Defense Enzyme Activity by Extraneous Factors to Fusarium oxysporum f.sp.melonisin Melon

作　　者：刘璐[1] 孙蕾[1] 张志鹏[1] 安美忱[1] 陈克农[1] 王学征[1] 

机构地区：[1]东北农业大学园艺学院,农业部东北地区园艺作物生物学与种质创制重点实验室,黑龙江哈尔滨150030

出　　处：《北方园艺》2016年第14期122-126,共5页Northern Horticulture

基　　金：国家自然科学基金资助项目(31372088)

摘　　要：以甜瓜抗病品系MR-1、感病品系M1-15为试材,以甜瓜枯萎菌为供试菌,采用营养钵栽培的方法,研究了水杨酸、茉莉酸甲酯和Ca^(2+)诱导对甜瓜幼苗枯萎病抗性的影响。结果表明:3种外源物质对甜瓜苗期枯萎病抗性的诱导效果不同,其中水杨酸和茉莉酸甲酯效果最好。1.0mmol·L^(-1)水杨酸诱导处理对甜瓜枯萎病的相对防效可达50.7%和45.7%;1.2mmol·L^(-1)茉莉酸甲酯诱导处理对甜瓜枯萎病的相对防效可达66.7%和40.3%,显著高于对照和Ca^(2+)处理;水杨酸、茉莉酸甲酯处理后,甜瓜植株叶片相关防御酶系多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性呈上升趋势。Taking two melon cultivars ＂MR-l＂and ＂M1-15＂ as materials, Fusarium oxysporum f. sp. meloris as test bacterial strain using the nutritional bowl of cultivayion methods, the effcet of different extraneous SA, MeJA, Ca2＋ induction resistance to Fusarium Wilt in melon were studied. The results indicated that the three exogenous substances had different effects on the resistance to Fusarium Wilt of melon,in which the effect of salicylic acid and methyl ester was the best. 1.0 mmol ·L-1 salicylic acid induced the relative control effect of 50. 7% and 45. 7% on the Fusarium Wilt of melon;1. 2 mmol ·L-1 methyl ester treatment on the relative prevention effect of melon wilt was 66.7% and 40. 3%, which was significantly higher than that of control and Caz＋ treatment. After induction of SA and MeJA,the activity of related resisitance enzyme polyphenol oxidase （PPO） and phenylalanine ammonia （PAL） in the leaf of melon was uptrend.

关 键 词：水杨酸 茉莉酸甲酯 Ca2+ 诱导抗性 枯萎病 防御酶 甜瓜 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]