刺五加茎总木脂素含量测定  

Determination of content of total lignans in the stem of acanthopanax senticosus

作　　者：张颖[1] 岳姝岐 李瑞芳[1] 孙晖[1] 

机构地区：[1]黑龙江中医药大学中药血清药物化学重点实验室,黑龙江哈尔滨150040

出　　处：《化学工程师》2016年第7期28-30,共3页Chemical Engineer

基　　金：国家科技支撑计划(2011BAI03B03)刺五加产业关键技术;综合开发利用研究

摘　　要：目的:建立紫外分光光度法测定刺五加茎中总木脂素含量的方法。方法:采用紫外分光光度法以刺五加苷E为对照品,在最大吸收波长处测定。结果:刺五加苷E显色后在560nm处有最大吸收,在6.93~23.1μg·mL^(-1)范围内呈良好线性关系(r=0.9995),平均回收率为99.28%(n=6)。结论:本方法可以快速准确的测定刺五加茎中总木脂素的含量。Objective：established A UV spectrophotometry method for the determination of total ignin in the stem of acanthopanax senticosus was. Method：Use ultraviolet spectrophotometer to measure total lignans in the stem of acanthopanax senticosus,eleutheroside E as reference substances, at the maximum absorption wavelength, respectively. Resuh：eleutheroside E with maximum absorption at 560nm,it had a good linear relationship within the range of 6.93～23.1μg·mL^-1 （r=0.9995）and the average recovery was 99.28% （n=6）. Conclusion：The method is simple ,accurate,and can be used for the quantitative determination of total ignin in the stem of acanthopanax senticosus.

关 键 词：刺五加 木脂素 含量测定 

分 类 号：R9[医药卫生—药学]