HPLC同时测定猫豆中左旋多巴及其衍生物含量  

Simultaneous Determination of L-dopa and Its Derivates in Seeds of Mucuna Pruriens Var. Utilis by HPLC

作　　者：邓霖芳 谭蓓蓓[1] 窦冕 郝丽莉[1] 刘江云[1] 杨世林[1] 

机构地区：[1]苏州大学医学部药学院,苏州215123 [2]滨州医学院附属医院,山东滨州256603

出　　处：《中国现代应用药学》2016年第7期845-848,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(81274190)

摘　　要：目的建立同时测定猫豆中左旋多巴（2）及其3种衍生物含量的高效液相色谱法。方法从猫豆中分离获得3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉（1）、1-甲基-3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉（3）、1,1二甲基-3-羧基-6,7-二羟基-1,2,3,4-四氢异喹啉（4）等3种成分;含量测定采用Cosmosil C18-PAQ色谱柱（4.6 mm×250 mm,5μm）,流动相1%乙酸等度洗脱,流速0.5 m L·min^-1,柱温30℃,检测波长280 nm。结果左旋多巴（2）和3个化合物色谱分离良好,分别在0.298 4-5.968μg（1）,6.554-131.1μg（2）,0.262 6-5.252μg（3）,0.256 8-5.136μg（4）线性关系良好（r〉0.999）,平均加样回收率依次为97.7%,99.1%,98.7%,97.9%。结论本方法简便、准确,可用于同时测定猫豆中的左旋多巴及其四氢异喹啉类衍生物含量,为其质量控制方法提供参考。OBJECTIVE To establish a method for simultaneous determination of both L-dopa（2） and its derivates in seeds of Mucuna pruriens var. utilis Burck by HPLC. METHODS Three ingredients, 3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline（1）, 1-methyl-3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline（3） and 1,1-dimethyl-3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline（4） were isolated from the herb. Chromatographic separation was performed on a Cosmosil C18-PAQ analytical column（4.6 mm×250 mm, 5 μm） with 1% acetic acid as the mobile phase at 30 ℃. The detection wavelength was 280 nm and the flow rate was 0.5 m L·min^-1. RESULTS The 4 components were separated with satisfaction. Their linearity ranges were between 0.298 4-5.968 μg（1）, 6.554-131.1 μg（2, L-dopa）, 0.262 6-5.252 μg（3） and 0.256 8-5.136 μg（4）, respectively with their correlation coefficients all 0.999. Their average recovery rates were 97.7%, 99.1%, 98.7% and 97.9%. CONCLUSION The method is accurate and selective for simultaneous determination of 4 ingredients in seeds of Mucuna pruriens, which could be applied for the quality control of this herb.

关 键 词：猫豆 左旋多巴 四氢异喹啉 高效液相色谱法 

分 类 号：R917.101[医药卫生—药物分析学]