微球透镜超分辨成像研究进展与发展前景  

Progress in and Prospect of Microsphere Optical Nanoscopy

作　　者：刘畅[1] 金璐頔 叶安培[1] 

机构地区：[1]北京大学纳米器件物理与化学教育部重点实验室,北京100871

出　　处：《激光与光电子学进展》2016年第7期19-31,共13页Laser & Optoelectronics Progress

基　　金：高等学校博士学科点专项科研基金(20120001110093);微生物资源前期开发国家重点实验室开放课题基金

摘　　要：受衍射极限的限制,传统光学显微镜的分辨率只能达到入射光波长的一半。超分辨显微镜已有很多,但制作工艺复杂,适用样品有限,对成像条件要求苛刻,因此应用受到很多限制。研究表明,将直径为几微米至几十微米的透明电介质微球置于样品表面,能显著提高传统光学显微镜的分辨能力,在白光下即可实现超分辨成像,与其他类型显微镜结合使用时也能保持超分辨能力。这种新型透镜为纳米结构和生物样本的实时超分辨成像提供了一种简单、直接的方式。结合本课题组研究结果,介绍并总结了国内外微球透镜的研究进展。The resolution of the conventional optical microscope is limited to about half of the incident wavelength because of diffraction limit. Although the super-resolution imaging is realized in many microscopes, their widespread applications are limited due to their complicated design, limited applicable samples and stringent requirement on imaging conditions. Recent researches show that the resolving ability of conventional optical microscopes can be improved significantly when the transparent dielectric microspheres with diameters from several to decades of microns are added on the sample surface, and thus super-resolution imaging with white light illumination is realized. The microspheres can keep their resolving ability when coupled with other types of microscopes. The microsphere nanoscope offers a simple direct way to realize real-time super-resolution imaging for nano-structures and biological samples. We review the research progress in the microsphere nanoseope at home and abroad with our own achievements combined.

关 键 词：成像系统 超分辨成像 微球透镜 光子纳米喷射效应 生物成像 

分 类 号：O439[机械工程—光学工程]