乙酰左旋肉碱对急性脊髓损伤大鼠细胞自噬、凋亡及运动功能的影响  

Effects of Acetyl-l-carnitine on Autophagy, Apoptosis, and Locomotor Function after Acute Spinal Cord Injury in Rats

作　　者：孟庆峰[1,2] 张明超[1] 卢伟[1] 毕云龙[1] 范仲凯[1] 

机构地区：[1]辽宁医学院附属第一医院骨科,辽宁锦州市121001 [2]抚顺市中心医院骨科,辽宁抚顺市113006

出　　处：《中国康复理论与实践》2016年第7期754-758,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：辽宁省高等学校优秀人才支持计划项目(No.LJQ2014091);辽宁医学院校长基金项目(No.XZJJ20130204)

摘　　要：目的旨在检测乙酰左旋肉碱(ALC)对大鼠急性脊髓损伤(SCI)后微管相关蛋白1轻链3(LC3)-Ⅱ、细胞凋亡及运动功能的影响。方法成年雌性Sprague-Dawley大鼠36只随机分为假手术组(Sham组)、单纯脊髓损伤组(SCI组)、ALC治疗组,每组12只。Allen法建立大鼠T10脊髓损伤模型。损伤后3 d行BBB运动评分;取脊髓组织,Western blotting和免疫荧光标记技术检测LC3-Ⅱ表达,TUNEL法观察细胞凋亡。结果与Sham组相比,SCI组LC3-Ⅱ明显升高(P<0.01),细胞凋亡显著增加(P<0.001),BBB评分显著降低(P<0.001);与SCI组相比,ALC组LC3-Ⅱ显著升高(P<0.001),细胞凋亡显著降低(P<0.001),BBB评分明显提高(P<0.01)。结论 ALC可能通过增强大鼠脊髓损伤后细胞自噬,减少细胞凋亡,促进大鼠运动功能恢复。Objective To observe the effects of acetyl-l-carnitine（ALC） on autophagy, apoptosis and motor function after acute spinal cord injury（ASCI） in rats. Methods Thirty-six adult female Sprague-Dawley rats were randomly divided into sham operation group（Sham group, n=12）, simple spinal cord injury group（SCI group, n=12）, ALC treatment group（ALC group, n=12）. Spinal cord injury model at the level of T10 segment was established using Allen＇s method. They were assessed with Basso-Beattle-Bresnahan（BBB） scale three days after injury. Then the rats were sacrificed, and the expression of microtubule associated protein 1 light chain 3（LC3）-Ⅱ in spinal cord was detected with Western blotting and immunofluorescent labeling, and the number of apoptotic cells were assessed with TUNEL staining. Results The expression of LC3-Ⅱ and the number of apoptotic cells increased in SCI group compared with those in Sham group（P〈0.01）, while the BBB score decreased（P〈0.001）. The expression of LC3-Ⅱ increased and the number of apoptotic cells decreased in ALC group compared with those in SCI group（P〈0.001）, while the BBB score increased（P〈0.01）. Conclusion ALC may promote autophagy, and inhibit apoptosis to improve the locomotor function after ASCI.

关 键 词：急性脊髓损伤 乙酰左旋肉碱 自噬 凋亡 大鼠 

分 类 号：R651.2[医药卫生—外科学]