褪黑素对脊髓损伤大鼠突触可塑性的作用  

Effects of Melatonin on Synaptic Plasticity after Spinal Cord Injury in Rats

作　　者：荆瀛黎[1,2,3,4] 刘小野[5] 白帆[1,2,3,4] 董浩[1,2,3,4] 陈惠[1,2,3,4] 

机构地区：[1]首都医科大学康复医学院,北京市100068 [2]中国康复研究中心中国康复科学所,北京市100068 [3]北京脑重大疾病研究院神经损伤与修复研究所,北京市100068 [4]神经损伤与康复北京市重点实验室,北京市100068 [5]首都医科大学附属北京友谊医院,北京市100050

出　　处：《中国康复理论与实践》2016年第7期774-778,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：北京脑重大疾病研究院科研促进项目(No.PXM2014_014226_000016)11421311;神经损伤与康复北京市重点实验室2015年度科技创新基地培育与发展专项项目(No.Z151100001615055);中央级公益性科研院所基本科研业务费专项资金项目(No.2015CZ-12)

摘　　要：目的探讨褪黑素对脊髓损伤后突触可塑性的影响。方法雌性Sprague-Dawley大鼠54只分为假手术组(n=18)、对照组(n=18)和褪黑素组(n=18)。采用改良Allen法复制大鼠T_(10)中度损伤模型(10 g×25 mm)。免疫荧光法检测运动神经元数目,尼氏染色检测神经元中尼氏小体表达;Western blotting检测神经纤维丝-200(NF-200)、脑源性神经营养因子(BDNF)、突触素Ⅰ和神经生长相关蛋白-43(GAP-43)的表达。结果术后7 d,与假手术组相比,对照组、褪黑素组运动神经元数、神经元中尼氏小体、NF-200、BDNF、突触素Ⅰ和GAP-43表达下降;与对照组相比,褪黑素组运动神经元数、神经元中尼氏小体、NF-200、BDNF、突触素Ⅰ和GAP-43的表达明显增加(P<0.01)。结论褪黑素能够修复损伤的突触可塑性,可能是促进运动功能恢复的机制。Objective To observe the effects of melatonin on synaptic plasticity impaired by spinal cord injury in rats. Methods A total of 54 female Sprague-Dawley rats were divided into sham group（n=18）, control group（n=18） and melatonin group（n=18）. Spinal cord injury model was established with modified Allen＇s method at T_（10）（10 g from 25 mm height）. The number of neurons and the expression of the Nissl body were detected with immunofluorescence and Nissl staining. The expression of neurofilament-200（NF-200）, brain-derived neurotrophic factors（BDNF）, Synapsin I and growth-associated protein-43（GAP-43） was detected with Western blotting. Results Seven days after injury, the number of motoneurons, the expression of Nissl body in motoneurons, and the expression of BDNF, Synapsin I and GAP-43 decreased in the control group compared with those in the sham group, and they increased in the melatonin group compared with those in the control group. Conclusion Melatonin can repair the impaired synaptic plasticity, which might promote the functional recovery after spinal cord injury.

关 键 词：脊髓损伤 突触可塑性 褪黑素 大鼠 

分 类 号：R651.2[医药卫生—外科学]