水中Mn(Ⅱ)对大菱鲆幼鱼生长及碱性磷酸酶和超氧化物歧化酶活性的影响  

Effects of Waterborne Mn(Ⅱ) on Growth and Activities of AKP and SOD in Juvenile Turbot(Scophthalmus maximus)

作　　者：何忠伟[1] 宫春光[1] 殷蕊 孙桂清 于骞[1] 符冬林 

机构地区：[1]河北农业大学海洋学院,河北秦皇岛066003 [2]河北省海洋与水产科学研究院,河北秦皇岛066200

出　　处：《水产科学》2016年第4期364-369,共6页Fisheries Science

基　　金：国家鲆鲽类产业技术体系项目(nycytx-50);河北省科学技术厅项目(12226748;14227111D);河北省教育厅项目(ZD20131062)

摘　　要：水温14～16℃下,将初始体质量为（5.0±1.0）g的大菱鲆养殖在40L水体的水族箱中,研究在不同Mn（Ⅱ）质量浓度（0.04、0.08、0.16、0.32、0.64、1.28、2.56、5.12、10.24mg/L）下大菱鲆的生长及碱性磷酸酶和超氧化物歧化酶的活性。结果表明,当水体中Mn（Ⅱ）质量浓度低于2.56mg/L时,鱼的生长较快,其中Mn（Ⅱ）质量浓度为0.64mg/L时生长最快;当Mn（Ⅱ）质量浓度低于0.64mg/L时,大菱鲆肝脏的碱性磷酸酶和超氧化物歧化酶活性随着Mn（Ⅱ）质量浓度的提高而增加,当Mn（Ⅱ）质量浓度超过0.64mg/L时,碱性磷酸酶和超氧化物歧化酶的活性急剧下降;0.64mg/L组的碱性磷酸酶和超氧化物歧化酶的活力均较高,随着Mn（Ⅱ）质量浓度增加,碱性磷酸酶和超氧化物歧化酶的活力先增后减;据剂量效应推测,0.64mg/L为Mn（Ⅱ）表现出毒性作用的临界质量浓度。In this study,growth and alkaline phosphatase（AKP）,and superoxide dismutase（SOD）activities were studied in juvenile turbot Scophthalmus maximus exposed to 0.04mg/L,0.08mg/L,0.16mg/L,0.32mg/L,0.64mg/L,1.28mg/L,2.56mg/L,5.12mg/L,and 10.24mg/L waterborne Mn（Ⅱ）.The results showed that better growth was observed in the turbot exposed to the waterborne Mn（Ⅱ）concentration of less than 2.56mg/L,the best growth at 0.64mg/L waterborne Mn（Ⅱ）.The AKP and SOD activities in the liver were shown to be increased with increasing Mn（Ⅱ）concentration,within less than0.64mg/L.At higher than 0.64mg/L in Mn（Ⅱ）concentration,however,AKP and SOD activities were decreased dramatically.During various treatment periods,the maximal AKP and SOD activities were observed in the turbot juveniles exposed to Mn（Ⅱ）concentration of 0.64mg/L,indicating that 0.64mg/L was inferred as the critical concentration to exhibit toxic effects according to dose-effect relation.The findings help to assess the toxicity of the water Mn（Ⅱ）to juvenile turbot,and provide the basis for aquaculture water management for Mn（Ⅱ）including determination of safe concentration.

关 键 词：锰 大菱鲆幼鱼 超氧化物歧化酶 碱性磷酸酶 

分 类 号：S965.399[农业科学—水产养殖]