基于线粒体DNA D-loop的都柳江鲇形目两种经济鱼类种群遗传多样性研究  

Genetic Diversity of 2 Economically Important Fishes in Siluriformes from Duliu River Based on mtDNA D-loop Sequences

作　　者：刘伟[1,2] 代应贵[1,2] 袁振兴[1,2] 孙际佳[3] 刘丽[3] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州大学特种水产研究所,贵州贵阳550025 [3]华南农业大学动物科学学院,广东广州510642

出　　处：《水产科学》2016年第4期386-392,共7页Fisheries Science

基　　金：国家公益性行业(农业)科研专项(201303048);贵州省留学人员科技活动项目[黔人项目资助合同(2013)10]

摘　　要：以采自贵州都柳江的鲇鱼、斑鳠各39尾个体为样品,采用PCR和DNA测序技术,研究了这两种鱼类线粒体DNA D-loop的结构和种群遗传多样性。获得了鲇鱼、斑鳠D-loop 5′端长度分别为544～545bp、546～547bp的DNA序列,并分别检测到58、15个变异位点。同时,识别了该2种鱼类D-loop终止序列区和中央保守区的核心序列,在中央保守区之后均有一个Poly-T结构。都柳江鲇鱼、斑鳠种群D-loop序列中,分别有51、12个多态位点,并分别定义了11、8种单倍型。都柳江鲇鱼、斑鳠种群单倍型多样性指数、核苷酸多样性指数和平均核苷酸差异数分别为0.772、0.02301、12.451和0.331、0.00131、0.714。目前,都柳江鲇鱼种群遗传多样性较为丰富,具有良好的种质价值和保护前景;斑鳠种群遗传多样性贫乏,保护和恢复该种群的数量和遗传多样性刻不容缓。The composition of mtDNA D-loop and genetic diversity were studied in 39 individuals of catfishes Silurus asotus and Mystus guttatus collected in the Guizhou section of Duliu River by the methods of PCR and DNA sequencing on the basis of sequences of mtDNA D-loop.There were 544—545bp and 546—547bp of D-loop sequences near the 5′end of D-loop,including 58 variable loci in S.asotus and 15 variable loci in M.guttatus.The characteristic sequences were identified in the extended termination associated sequence and the central conserved domain and a poly-T next to the 3′end of central conserved domain in the D-loop sequence in each of the two species.There were 51 and 12polymorphic loci and 11 and 8haplotypes in the populations of S.asotus and M.guttatus,respectively.S.Asotus had haploidtype diversity of0.772,nucleotide diversity of 0.02301,and average nucleotide differences of 12.451,and M.guttatus had haploidtype diversity of 0.331,nucleotide diversity of 0.00131,and verage nucleotide differences of 0.714.The population of S.asotusfrom the Duliu River showed abundant genetic diversity,good germplasm value and protection outlook.While the population of M.guttatus had much low genetic diversity in the Duliu River.Therefore,it is urgent to protect and recover the genetic diversity of M.guttatus population from the Duliu River.

关 键 词：都柳江 鲇鱼 斑鳠 D-LOOP 遗传多样性 

分 类 号：S917[农业科学—水产科学]