反复冻融对血浆抵抗素和内脂素测定水平的影响  

Effects of Repeated Freeze- thaw Cycles on Plasma Resistin and Visfatin Concentration

作　　者：张林峰[1] 李莹[1] 郭敏[1] 田野[1] 赵连成[1] 

机构地区：[1]中国医学科学院/北京协和医学院国家心血管病中心、阜外心血管病医院、心血管疾病国家重点实验室、社区防治部,100037

出　　处：《医学研究杂志》2016年第8期17-20,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(30901237)

摘　　要：目的探讨反复冻融对血浆抵抗素和内脂素测定水平的影响。方法抽取-70℃保存的血浆标本22份,化冻后用酶联免疫吸附法(ELISA)检测血浆抵抗素和内脂素水平(冻融1次标本),同时将每份标本分装2管,一管放入-20℃冷冻后取出,室温融解,重复3次(冻融4次标本);另一份按相同方法重复5次(冻融6次标本),用同一试剂盒、在同一批内检测血浆抵抗素、内脂素水平。比较3种冻融方式间的差异。结果标本在冻融1次、4次和6次的情况下,抵抗素测定水平分别为2.24±0.99、2.01±1.05、1.89±1.01ng/ml,内脂素测定水平分别为16.3±7.6、13.4±6.3、12.1±5.6ng/ml,后两次检测抵抗素水平较第1次分别下降10.3%(t=5.05,P=0.000)和15.6%(t=10.65,P=0.000);内脂素水平较第1次检测分别下降17.8%(t=5.19,P=0.000)和25.8%(t=6.58,P=0.000)。结论反复冻融对血浆标本中抵抗素和内脂素的水平有显著影响,提示在血标本贮存运输、测定和分析中要注意这一现象对研究结果的影响,避免标本的反复冻融。Objective To assess the effect of repeated freeze - thaw cycles on plasma resistin and visfatin concentration. Methods Plasma samples stored at -70 degrees C were obtained from 22 patients and assayed for resistin and visfatin using enzyme linked immunosorbent assay （ELISA）. Following baseline measurement, the samples were divided into two groups for each patient and assayed again, one group experienced 3 times of freeze and thaw cycles and the other group experienced 5 times of freeze and thaw cycles. Paired t test was used to test the significance of changes from baseline. Results The concentration of resistin for the three tests were 2.24 ±0.99ng/ ml, 2.01 ± 1.05ng/ml and 1.89 ±1.01ng/ml, respectively. Compared with baseline, the concentration of the second test decreased 10. 3 % （ t = 5.05, P = 0. 000 ） and the concentration of the third test decreased 15.6% （ t = 10.65, P = 0. 000 ）. The concentration of visfatin for the three tests were 16.3 ±7.6ng/ml, 13.4 ± 6.3ng/ml and 12.1 ± 5.6ng/ml, respectively. Compared with baseline, the concentration of the second test decreased 17.8% （t =5.19,P =0. 000） and the concentration of the third test decreased 25.8% （t =6.58,P = 0. 000）. Conclusion Freeze - thaw cycle has a significant effect on the plasma resistin and visfatin concentration and could lead to the decrease of the results, which should be taken into account in the process of storage, ship and assaying the blood samples.

关 键 词：冻融 血浆抵抗素 血浆内脂素 酶联免疫吸附法 

分 类 号：R3[医药卫生—基础医学]