核因子-κB在放射性心脏损伤中的表达  

Expression of Nuclear factor-κB in Radiation Induced Heart Disease

作　　者：姜敏[1] 曾越灿[1] 高嵩[1] 吴荣[1] 

机构地区：[1]中国医科大学附属盛京医院肿瘤科,沈阳110022

出　　处：《医学研究杂志》2016年第8期36-40,共5页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81201803)

摘　　要：目的核因子-κB在放射性心脏损伤中的表达及机制探讨。方法共32只SD大鼠,随机将大鼠分为3组,1组不接受任何干预,其余2组分别用6Mv高能X线照射心脏,剂量分别为15Gy和18Gy。照射后饲养3个月后,以水合氯醛过量麻醉法处死大鼠,取其心脏组织。进行心肌组织HE染色及Masson染色,观察心肌变化,通过核因子-κB的免疫组化染色,显微镜下观察核因子-κB大致的表达情况并使用计算机测定其平均光密度值。结果 HE染色镜下观察照射组心肌细胞排列紊乱,部分细胞出现变性及坏死,细胞核排列不规则,对照组心肌细胞排列正常。Masson染色镜下可见照射组心肌内胶原组织明显增多,粗大胶原及纤维相互连接成网状,纤维化明显,对照组未见明显改变。免疫组化镜下观察可见对照组核因子-κB有少量表达,照射15Gy组、照射18Gy组的表达均增强(P值分别为0.002、0.000)。但两照射组之间比较差异无统计学意义(P=0.095)。结论核因子-κB是放射性心脏疾病发生、发展的重要因素,其表达不随照射剂量增大而增强。Objective To discuss the expression and mechanism of nuclear factor -κB in radiation - induced heart disease. Methods Forty Sprague Dawley（SD） rats were divided into 3 groups： the control group, 15Gy radiation group and 18Gy radiation group. Experimental animals were exposed to radiation（ X -rays） generated by a linear accelerator at the chest and sacrificed by chloral hydrate overdose method after 3 months and heart was removed. Results HE staining showed disorder arrangement of myocardial cells in the radiation group, and degeneration and necrosis could also be observed. Nuclei arranged irregularly, myocardial cells arranged normally in the control group. Masson staining showed normal morphology of the myocardium in the control group and no sign of fibrosis. On contrary, myocardium of the radiation groups had significantly higher collagen content, thick collagen fibers interconnected, all of which were sign of fiberosis. Immunohistochemical staining showed that little NF - KB expression was detected in myocardium of the control group while abundant NF - KB expression was detected of 15 Gy and 1SGy radiation groups （P =0. 002,P = 0. 000, respectively）. And there was no significant difference between the two irradiation groups（P = 0. 095）. Conclusion Nuclear factor -κB is an important factor that can cause radiation -induced heart disease. As the radiation dose rises, the expression content does not change.

关 键 词：放射性心脏损伤 核因子-ΚB 

分 类 号：R318[医药卫生—生物医学工程]