类风湿关节炎患者B细胞上Fas受体表达与疾病活动度相关性研究  

Correlation Between Expression of Fas Receptor Molecules on B Lymphocyte and Disease Activity of Rheumatoid Arthritis

作　　者：王鑫[1] 魏平[2] 

机构地区：[1]绍兴市人民医院内分泌代谢免疫风湿科,312000 [2]河北医科大学第三附属医院免疫风湿科,石家庄050000

出　　处：《医学研究杂志》2016年第8期138-141,共4页Journal of Medical Research

摘　　要：目的测定类风湿关节炎(RA)患者B淋巴细胞表面Fas及可溶性Fas/Fas L(s Fas/s Fas L)的表达,研究B淋巴细胞Fas受体在RA发病中的作用。方法流式细胞术检测CD20+标记的B淋巴细胞及Fas的表达,酶联免疫吸附试验检测s Fas/s Fas L的水平。结果 RA组血清及关节液s Fas/s Fas L水平均高于对照组(P<0.01),RA组外周血Fas表达率高于对照组(P<0.01),CD20+B淋巴细胞表面受体Fas表达率低于对照组(P<0.01)。结论 RA中s Fas、s Fas L表达升高,可能通过抑制致病淋巴细胞凋亡,促进RA发病;RA发病中T/B淋巴细胞比例紊乱,外周血淋巴细胞Fas升高而B淋巴细胞Fas表达降低,可能是由s Fas/s Fas L升高引起。Objective To investigate the role of B lymphocyte and Fas receptor mediated apoptosis in the pathogenesis of rheumatoid arthritis（RA） through detecting the count of peripheral B lymphocytes, apoptosis factor of Fas expression and the level of soluble Fas/ FasL （sFas/sFasL） in serum and synovial fluid apoptosis index. Methods The percentage of CD20 ＋ in B lymphocytes and apoptosis factor of Fas in peripheral blood were detected with flow cytometry（FCM） their correlation with clinical index was and analyzed. Serum and synvial fluid levels of sFas/sFasL were determined by enzyme - linked immunosorbent assay （ELISA） and their correlation with clinical index was amalyzed. Results The level of sFas/sFasL in the serum and synvial fluid in RA patients were higher than that in normal con- trois （ P 〈 0.01 ）. The prevalence of Fas cell in peripheral blood of RA patients was significantly higher than normal controls （ P 〈 0.01 ）. The prevalence of CD20＋ in B lymphocytes and Fas receptors of B lymphocyte surface in peripheral blood of RA patients was significantly lower than normal controls （P 〈 0.01 ）. Conclusion Increasing expression of sFas and sFasL in RA may be promote RA disease by inhibiting pathogenic lymphatic cell apoptosis. B lymphocytes were reduced in RA lead to T/B lymphocytes dysfunction and breaking the mechanisms of immune system. Fas rose in peripheral blood lymphocyte and the B lymphocytes of Fas is reduced, which may be caused by the hoist of sFas/sFasL.

关 键 词：类风湿关节炎凋亡CD20 CD95 B淋巴细胞 

分 类 号：R593.22[医药卫生—内科学]