小花鬼针草化学成分液质联用分析  

Liquid Chromatography-mass Spectrometry Analysis of Chemical Constituents in Bidens parviflora Willd.

作　　者：李胜峰[1] 刘阿娜[1] 蒋海强[1] 张玲[1] 周洪雷[1] 

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《山东中医杂志》2016年第7期641-644,共4页Shandong Journal of Traditional Chinese Medicine

基　　金：2013年山东省科技发展计划项目(编号:2013GSF11903);山东中医药大学2014年度大学生创新训练项目(编号:2014141)

摘　　要：目的:建立小花鬼针草化学成分的定性表征方法。方法:采用高效液相色谱-电喷雾-飞行时间质谱联用技术,色谱柱为HALO C18(150 mm×3.0 mm,2.7μm),流动相为0.1%甲酸水溶液-乙腈(梯度洗脱),采用化学成分数据库构建-液质数据采集-数据库检索-质谱数据验证的方法鉴定主要化学成分。结果:鉴定出小花鬼针草化学成分18种,主要包括Chalcone,α,3,2’,4’-tetrahydroxy-2'-O-β-D-glucopyranosyl、Rutin、Luteolin 7-O-β-D-glucopyranoside等黄酮类化合物和Chlorogenic acid、3,4-Di-Ocaffeoylquinic acid、3,5-Di-O-caffeoylquinic acid等有机酸类。结论 :液质联用技术能够有效鉴定小花鬼针草中的化学成分。Objective：To establish a method for the qualitative characterization of chemical constituents of Bidens parviflora Willd. Method：High performance liquid chromatography-electrospray-time of flight mass spectrometry（HPLC-ESI-TOF/MS） was applied with HALO Cis（150 mmx3.0 mm,2.7 μm） column and mobile phase, 0.1% formic acid-acetonitrile （gradient elution）. Chemical ingredients were identified through building chemical constituents database-LC-MS data acquisition-database search-mass data validation method. Result： There were 18 chemical ingredients were identified mainly including flavonoids （chalcone,α,3,2＇,4＇-tetrahy- droxy-2＇-O-β-D-glucopyranosyl,rutin,luteolin 7-O-β-D-glucopyranoside and so on） and organic acids （chloro- genie acid, 3,4-Di-O-caffeoylquinic acid,3,5-Di-O-caffeoylquinic acid and so on）. Conclusion ：Liquid chro- matography-mass spectrometry is effective for identifying chemical ingredients of Bidens parviflora Willd.

关 键 词：小花鬼针草 化学成分 高效液相色谱-电喷雾-飞行时间质谱联用技术 

分 类 号：R284.1[医药卫生—中药学]