多杀性巴氏杆菌和猪圆环病毒混合感染的诊断  

Diagnosis of the combined infection with Circovirus and Pasteurella of swine

作　　者：马莉莉[1] 王宇婷[1] 赵微[1] 翟军军[1] 倪宏波[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《现代畜牧兽医》2016年第7期36-39,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：博士科研启动基金(编号:LBH-Q14134)

摘　　要：大庆市某猪场发生以高热稽留、呼吸困难以及食欲废绝等为临床症状,部分仔猪甚至死亡的疫情。无菌采集病料,提取病变组织DNA或RNA进行猪瘟病毒、圆环病毒的PCR或RT-PCR检测,同时进行细菌分离培养等试验。PCR扩增出700 bp的猪圆环病毒特异性条带。诊断为多杀性巴氏杆菌和猪圆环病毒混合感染。A pig farm with fever, difficulty breathing and loss of appetite as the clinical symp- toms in the city of Daqing, some pigs even death. Collected aseptically diseased tissue extract DNA or RNA for PCR or RT-PCR detecting circovirus and swine fever virus, while for bacterial iso- lation tests. PCR amplified 700 bp porcine circovirus specific bands. By laboratory diagnosis a pig farm pigs died, the etiology of the disease is diagnosed with Pasteurella and Circovirus com- bined infection.

关 键 词：圆环病毒 巴氏杆菌 混合感染 诊断 

分 类 号：S858.28[农业科学—临床兽医学]