鹿瓜多肽关节腔注射治疗家兔类风湿性关节炎实验研究  

Experimental Study on Treatment of Rheumatoid Arthritis in Rabbits by Injection of Deer Melon Polypeptide

作　　者：冯艳琴[1] 敖金波[2] 杨敏[1] 程建明[2] 陈德森[3] 

机构地区：[1]十堰市太和医院湖北医药学院附属医院骨科,十堰442000 [2]十堰市太和医院湖北医药学院附属医院康复中心,十堰442000 [3]十堰市太和医院湖北医药学院,十堰442000

出　　处：《实验动物与比较医学》2016年第3期204-207,共4页Laboratory Animal and Comparative Medicine

基　　金：湖北省教育厅重点项目（B20122406）

摘　　要：目的 研究鹿瓜多肽治疗家兔类风湿性关节炎（RA）的机制.方法 用小牛软骨Ⅱ型胶原＋弗氏完全佐剂0.1mL于家兔左膝关节腔内多点注射的方法制备RA模型,模型动物分为模型组及低、高剂量鹿瓜多肽（1 mg/d、2 mg/d）治疗组（A、B组）,分别计算治疗后1d、7d、14 d、21d足肿胀度,测定关节积液中透明质酸（HA）、白细胞介素-1p（IL-1p）,基质金属蛋白酶（MMP-3）及骨形态发生蛋白（BMP）含量.结果 与模型组比较,鹿瓜多肽低、高剂量组14 d、21d时动物足肿胀度、MMP-3、IL-1β含量明显降低,BMP、HA含量明显升高（P＜0.05）,低、高剂量组间无明显差异（P＞0.05）.结论 鹿瓜多肽可提高家兔关节腔HA合成及BMP分泌,抑制MMP-3及IL-1β对关节滑膜的浸蚀而达到治疗RA的目的.Objective To study the mechanism of the treatment on rheumatoid arthrist （RA） in rabbits by deer melon polypeptide. Methods Calf cartilage II collagen＋Freund＇s complete adjuvant （0.1 mL） was injected intra-articular in right knee of rabbits to prepare RA model. The model rabbits were randomly divided into model group, low and high dosage deer melon polypeptide group （Group A and B） with 1 mg/d or 2 mg/d. The paw swelling were calculated at 1, 7, 14, 21 days after treatment, the hyaluronic acid （HA）, interleukin- 1β （IL- 1β）, matrix metalloproteinase （MMP-3） and bone morphogenetic protein （BMP） content in joint effusion were measured. Results Compared with the model group, paw swelling were occurred in the rabbits of group A and B, MMP-3 and IL- 1 β were significantly decreased, BMP and HA significantly increased（P〈0.05）, but no difference between groups A and B （P〈0.05）. Conclusion The deer melon polypeptide had the treatment effects on RA in rabbit by improving the RA synthesis and BMP secretion in articular, and inhibiting the erosion of MMP-3 and IL- 1β on synovial.

关 键 词：家兔 类风湿性关节炎(RA) 鹿瓜多肽 

分 类 号：R593.22[医药卫生—内科学] R-332[医药卫生—临床医学]