麻黄素对小鼠肺组织结构的影响  

Effects of ephedrine on histological structure and its mechanism in the mouse lung

作　　者：彭静[1] 李重阳[2] 刘婷婷[1] 俞诗源[1,3] 

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]甘肃政法学院公安分院,兰州730070 [3]西北师范大学学报编辑部,兰州730070

出　　处：《解剖学报》2016年第4期521-527,共7页Acta Anatomica Sinica

基　　金：甘肃省自然科学基金(1107RJZA141);兰州市社会发展项目(2013-3-72;2014-1-105)

摘　　要：目的探讨麻黄素对小鼠肺组织的影响。方法 60只小鼠随机分为麻黄素组和对照组,采用递增剂量腹腔连续注射麻黄素溶液(0.0315 g/kg、0.0477 g/kg、0.0564 g/kg)和等量生理盐水15d(每天两次,每次0.2ml),用比色法检测各组小鼠肺组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量及血浆髓过氧化物酶(MPO)活性的变化,用苏木素-伊红染色法观察肺组织结构的变化,用免疫组织化学法检测肺组织c-Fos蛋白和Caspase-3蛋白表达的变化。结果麻黄素组小鼠肺组织SOD、CAT活性5d时暂时升高,10d、15d时显著降低,MDA含量5d时暂时降低,10d、15d时显著升高;血浆MPO活性显著升高,肺组织病理损伤明显,肺泡直径和肺泡隔厚度显著增大,放射状肺泡数(RAC)明显减小;肺组织c-Fos、Caspase-3蛋白阳性表达的平均吸光度值明显增强。结论麻黄素可加重机体炎症反应,影响小鼠肺组织抗氧化酶活性,诱导肺组织细胞凋亡,导致小鼠肺的组织结构和功能受损。Objective To explore effects of ephedrine in the mouse lung. Methods Sixty mice were randomly divided into control group and ephedrine group. The mice in ephedrine group were injected with ephedrine at the doses0. 0315 g / kg,0. 0477 g / kg,0. 0564 g / kg（ × 0. 2ml）,respectively,twice per day for 15 days,and the mice in control group were injected with equal amount of saline. Changes in the activity of superoxide dismutase（ SOD）,catalase（ CAT） and the content of maleicdialdehyde（ MDA） in mice lung and the activity of plasma myeloperoxidase（ MPO） were analyzed by colorimetry. Hematoxylin-eosin staining was used to observe the changes of histological structures in the mouse lung. The expressions of c-Fos protein and Caspase-3 protein were examined by immunohistochemical method. Results The activities of SOD,CAT were higher temporarily in the ephedrine group than those in the control group at the fifth treatment day,but they were significantly lower in the ephedrine group than those in the control group at the 10 th and 15 th treatment days.MDA content was reduced temporarily at the 5th treatment day,while were significantly increased at the 10 th and 15 th treatment days. The activity of plasma MPO increased. Histopathologic damage in the lung of ephedrine group was obvious.The alveolar diameter and thickness of the alveolar septum increased and radical alveolar counts（ RAC） reduced significantly. The intensities of immunohistochemical positive staining for c-Fos,Caspase-3 proteins were increased significantly in the lung. Conclusion Ephedrine significantly affects the antioxidant enzyme activity,increases the inflammatory reaction of body,induces cell apoptosis,and damages histological structures and function in the mouse lung.

关 键 词：麻黄素 肺 抗氧化酶 组织结构 C-FOS蛋白 CASPASE-3蛋白 免疫组织化学 小鼠 

分 类 号：R338.21[医药卫生—人体生理学]