中国广东地区汉族人群P选择素糖蛋白配体-1Met62Ile基因多态性与冠心病的相关性探讨  

Investigate the relationship between P-selectin glycoprotein ligand-1 M62I gene polymorphism and coronary heart disease in China Guangdong Han Population

作　　者：明凯华[1] 雷秀霞[1] 邹愉[1] 杨娜[1] 徐邦牢[1] 黄子宜[1] 

机构地区：[1]广州市第一人民医院检验科,广东广州510180

出　　处：《分子诊断与治疗杂志》2016年第4期237-241,共5页Journal of Molecular Diagnostics and Therapy

基　　金：广州市卫生医药科技项目(20151A010009)

摘　　要：目的探讨P选择素糖蛋白配体-1(P-selectin glycoprotein ligand-1,PSGL-1)Met62Ile(M62I)基因多态性在中国广东地区汉族人群中的分布特点及其与冠心病(coronary heart disease,CHD)的相关性。方法应用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction restrictionfragment length polymorphism,PCR-RFLP)分析方法检测200名该地区冠心病患者和200名汉族健康人群PSGL-1 M62I基因型,计算并比较各组基因型频率及等位基因频率。结果 200名冠心病组中P选择素PSGL-1 M62I基因型以Met/Met(M/M)型发生频率最高(73.0%),Met/Ile(M/I)型次之(27.0%),未检测到Ile/Ile(I/I)型;M、I各型等位基因频率分别为86.5%、13.5%,该位点基因多态性分布在男女间无显著性差异(P>0.05)。200名正常对照组中P选择素PSGL-1 M62I基因型以M/M型发生频率最高(69.0%),M/I型次之(31.0%)未检测到I/I型;M、I各型等位基因频率分别为84.5%、15.5%,该位点基因多态性分布在男女间无显著性差异(P>0.05)。两组人群间各基因型及等位基因分布无显著性差异(P>0.05)。结论中国广东地区冠心病患者和健康人群PSGL-1 M62I基因多态性在男女之间均无较大的差异。PSGL-1 M62I位点可能不是冠心病的易感基因。Objective To investigate the different age groups distribution of P-selectin glycoprotein ligand- 1（PSGL- 1） Met62Ile（M/I） gene polymorphism in the Chinese Han population and the relationship between the polymorphism and coronary heart disease（CHD）. Methods Polymerase chain reaction restriction fragment length polymorphism（PCR- RFLP） analysis was used to detect the genotype and allele frequency of PSGL- 1 M62 I in 200 patients with CHD and then the genotype and allele frequencies were compared to a control population of 200 healthy, Han Chinese. Results In the 200 CHD patients of Guangdong area, the highest frequency of PSGL-1 M62 I genotype is M/M（73.0%）, followed by M/I（27.0%）. The I/I genotype was not detected; M, I allele frequencies were 86.5% and 13.5%. These gene polymorphisms had no significant difference between males and females（P〉0.05）. In the healthy control population, the highest frequency of PSGL-1 M62 I genotype is M/M（69.0%）, followed by M/I（31.0%）. The I/I genotype was also not detected. The allele frequencies of M and I were 86.75% and 13.25%. These gene polymorphisms had no significant difference between males and females（P〉0.05）. There were no significant differences between CHDgroup and normal group（P〉0.05）. Conclusion Between the CHD patients and healthy Han Chinese population of the Guangdong area in China, there were no significant differences in PSGL- 1 M62 I gene polymorphism. The PSGL-1 M62 I gene may not be the susceptibility gene of CHD.

关 键 词：PSGL-1 基因多态性 冠心病 性别 

分 类 号：R541.4[医药卫生—心血管疾病]