表达H9亚型禽流感病毒血凝素蛋白重组新城疫病毒耐热株的构建  

Construction of the Recombinate Thermostable Newcastle Disease Virus Expressing Hemagglutinin Protein of H9 Subtype Avian Influenza Virus

作　　者：胡潇[1,2] 温国元[2] 王红琳[2] 罗青平[2] 邵华斌[2] 潘兹书[1] 

机构地区：[1]武汉大学生命科学学院/病毒学国家重点实验室,湖北武汉430072 [2]湖北省农业科学院畜牧兽医研究所,湖北武汉430064

出　　处：《武汉大学学报（理学版）》2016年第4期345-349,共5页Journal of Wuhan University:Natural Science Edition

基　　金：国家现代农业产业技术体系建设专项资金(CARS-43-G11);公益性行业(农业)科研专项基金(201303033);湖北省农业科学院青年基金(2015NKYJJ10)

摘　　要：应用反向遗传操作技术,将H9亚型禽流感病毒血凝素(HA)蛋白基因替换重组新城疫病毒耐热rTS-GFP/M株的绿色荧光蛋白基因,构建表达H9亚型禽流感病毒HA蛋白的重组新城疫病毒rTS-HA/M株.蛋白表达检测和病毒特性分析结果表明,该重组病毒能够在BHK-21细胞中高效表达HA蛋白,具有与亲本毒株类似的增殖能力、耐热特性和低致病性.To construct the recombinant newcastle disease virus（NDV）strain rTS-HA/M expressing the H9 subtype avian disease virus（AIV）hemagglutinin（HA）protein,the green fluorescence protein（GFP）gene in the recombinant NDV thermostable strain rTS-GFP/M was replaced by the H9-AIV HA gene using the reverse genetic technology.The HA expression of rTS-HA/M was analyzed by the immune-fluorescence assay and western blot,and the recombinant strain rTS-HA/M was characterized.The results demonstrated that the recombinant NDV strain rTS-HA/M efficiently expressed AIV-H9 HA protein in BHK-21 cells,and that the proliferation ability,thermostability and low pathogenicity of rTS-HA/M were maintained compared with its parental stain.

关 键 词：H9亚型禽流感 新城疫病毒 耐热株 血凝素 二联疫苗 

分 类 号：S852.659.5[农业科学—基础兽医学]