猪TGFBR1基因的克隆与生物信息学分析  

Cloning and bioinformatic analysis of TGFBR1 gene in the Erhualian pig

作　　者：周吉隆[1] 姚望[1] 刘红林[1] 李齐发[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《畜牧与兽医》2016年第7期21-27,共7页Animal Husbandry & Veterinary Medicine

基　　金：江苏省农业科技自主创新资金(SCX(12)2097)

摘　　要：TGFBR1是TGF-β/Smad信号通路中的重要成员,在哺乳动物卵泡发育和排卵过程中发挥重要作用。本文通过克隆测序技术分离猪TGFBR1基因编码区序列,采用生物信息学方法对猪TGFBR1序列信息及其与哺乳动物其他物种间的进化和系统发育关系进行分析。结果发现:猪TGFBR1基因编码区序列全长1 512 bp,编码蛋白含有503个氨基酸残基,与哺乳动物其他物种的一致性分别在90%和95%以上;猪TGFBR1蛋白具有跨膜结构、GS区和激酶结构等保守结构域。进化分析显示哺乳动物TGFBR1基因的突变已达饱和状态,在进化过程中受到负选择的影响。基于TGFBR1基因编码区序列的系统发育分析发现,猪与同属偶蹄目的普通牛、绵羊的亲缘关系较近。TGFBR1 is an important member of the TGFβ / smad signaling pathway. It plays a vital function in the process of follicular development and ovulation in mammals. In this paper,the coding sequence of TGFBR1 in the Erhualian pig was obtained by cloning and sequencing techniques. Bioinformatics method was used to analysis the TGFBR1 sequence information. The evolution and phylogenetic relationship between Erhualian pig and other species of mammals in TGFBR1 was also analyzed. The result shows that the length of TGFBR1 coding sequence in the Erhualian pig was 1512 bp,which encoding 390 amino acids. By comparison,the consistency of Erhualian pigs and mammals or other species were 90% and 95% or more in TGFBR1. The Erhualian pig TGFBR1 included some conserved regions such as transmembrane,GS and kinase. Phylogenetic analysis showed the mutation of TGFBR1 gene in mammals had reached saturation. It was selected by negative effect in the evolutionary process. Also,it revealed Erhualian pigs have closer relationship with cattle and sheep belong to hoofed.

关 键 词：猪 TGFBR1基因 克隆 生物信息学分析 

分 类 号：S828[农业科学—畜牧学]