提取方法对地衣提取物抗肿瘤活性的影响  

Influence of Different Extraction Methods on Antitumor Activity of Usnea diffracta Vain Fraction

作　　者：胡松杉 贺小琼[1] 吴怡[2] 姚乾[3] 王静[1] 贺云发 

机构地区：[1]昆明医科大学公共卫生学院 [2]昆明医科大学科研实验中心,昆明650500 [3]昆明医科大学第三附属医院云南省肿瘤研究所,昆明650031

出　　处：《天然产物研究与开发》2016年第7期1102-1108,共7页Natural Product Research and Development

基　　金：国家自然科技基金(30960437);云南省科技计划项目(2009FXL001);云南省科技厅-昆明医科大学联合专项重点项目(2012FB001)

摘　　要：采用60%乙醇常温、60%乙醇70℃水浴,95%乙醇常温、95%乙醇70℃水浴、95%甲醇常温和95%甲醇70℃水浴6种手段对某地衣植物进行提取,粗提物再经80%乙醇醇沉和氯仿萃取,得到研究样品A、B、C、D、E、F。比较不同提取条件下样品的得率、体外抗肿瘤活性和色谱图差异,与已分离的功效化合物图谱进行比较,筛选较优的活性部位提取工艺。结果表明,乙醇相对于甲醇能够更有效提取功效化合物a和b。60%乙醇70℃恒温水浴提取的有效部位得率为1.01%,在40μg/m L浓度下对Hela和T-24的抑制率分别为81.17%、88.56%,体外抗肿瘤活性最强,为较优的抗肿瘤活性部位提取方法。Six kinds of extraction methods, including 60% ethanol at room temperature, 60% ethanol at 70 ℃ water- bath,95% ethanol at room temperature,95% ethanol at 70℃ water-bath,95% methanol at room temperature,95% methanol at 70 ＇E water-bath were used for the extraction of Usnea diffracta Vain fraction. The crude extracts were then further extracted by 80% ethanol, and the ethanol extracts were finally fractionated by chloroform to obtain sample A, B, C, D,E and F, respectively. The yield, antitumor activity and HPLC chromatograms of 6 samples were then compared. The HPLC chromatograms of 2 purified bioactive compounds （compound a and b）were used for the identification of bio- active peaks. Our results showed that ethanol, not methanol, can effectively extract bioactive compounds a and b. Sample B showed the highest inhibitory rates both against Hela and T-24 tumor cell lines（81.17% and 88.56% at 40 μg/mL, respectively）. Present study indicated that extraction method and extraction solvent significantly influenced the bioactivi- ty of sample. 60% ethanol at 70℃ water-bath was the optimal method for the extraction of the antitumor bioactive frac- tion among the 6 methods.

关 键 词：提取工艺 抗肿瘤活性 有效组分 高相液相色谱 

分 类 号：R284.2[医药卫生—中药学]