含银海藻酸盐敷料理化性质及其抗菌性能的研究  

Physico-chemical properties and antimicrobial activity of silver ion dressings

作　　者：李小军[1,2,3] 顾其胜 王庆生 李俊杰[2] 张琪[1] 

机构地区：[1]武警总医院,北京100039 [2]军事医学科学院基础医学研究所,北京100850 [3]厦门海沧李小军口腔诊所,福建厦门361026 [4]上海其胜生物材料技术研究所,上海200621 [5]山东颐诺生物科技有限公司,山东滨州256500

出　　处：《军事医学》2016年第7期564-568,共5页Military Medical Sciences

摘　　要：目的探索含银海藻酸盐敷料的理化性质、生物安全性及抗菌活性。方法通过扫描电子显微镜(SEM)等实验方法对含银离子海藻酸盐敷料进行形貌及理化特征分析;通过MTT法,检测敷料的生物安全性;结合体外抑菌实验,研究敷料的抗菌效果。结果与海藻酸钙敷料相比,3种不同含量的银离子敷料透气率无显著差异(P>0.05);银离子浓度越高,其溶胀比率越高,吸水能力越强,降解越慢;MTT结果显示,细胞毒性较低;体外抑菌实验证实,银离子敷料对革兰阳性、阴性菌均具有良好的抑菌作用,同时显示银离子浓度越高,其抑菌效果越好。结论含银海藻酸盐敷料具有良好的生物相容性和抗菌活性,在创面愈合治疗中具有潜在的应用前景。Objective To detect the physico-chemical properties, cytotoxicity and antimicrobial activity of dressings containing Ag ＋. Methods The morphology of this dressing was shown by the scanning electron microscope （SEM）. The cytotoxicity was detected by 3-（4,5-dimethyhhiazol-2-yl） -2,5-diphenyltetrazolium bromide（MTF） method. Using the shake flask test method, the antibacterial effect of dressings was studied. Result There was no significant difference in the water vapor transmission rate between the dressings（P 〉 0.05 ）. Compared with the alginate calcium dressing, silver ion dressings had a stronger swelling rate. A higher ion concentration would lead to a much larger swelling rate and slower degradation. A lower cytotoxicity was exhibited among the dressings. The silver ion dressing had stronger bacteriostasis to Gram-positive （G＋ ） and Gram-negative（G- ） bacteria than the alginate calcium dressing. Conclusion The experiment has proved that the silver ion dressing has stronger antibacterial activity and lower cytotoxicity, and it is more effective for wound surface healing, with a shortened treatment course.

关 键 词：生物敷料 银离子 海藻 细胞毒性 抗菌活性 显微镜检查 电子 扫描 

分 类 号：R318.08[医药卫生—生物医学工程]