Effect of Ligustrazine Nanoparticles Nano Spray on Transforming Growth Factor-β/Smad Signal Pathway of Rat Peritoneal Mesothelial Cells Induced by Tumor Necrosis Factor-α  

作　　者：颜帅 杨斓 乐音子 李文林 曾莉 乐进 毛春芹 

机构地区：[1]Suzhou Hospital of Traditional Chinese Medicine [2]First Clinical Medical College of Nanjing University of Chinese Medicine [3]Library of Nanjing University of Chinese Medicine [4]Yancheng Hospital of Traditional Chinese Medicine [5]College of Pharmacy of Nanjing University of Chinese Medicine

出　　处：《Chinese Journal of Integrative Medicine》2016年第8期629-634,共6页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.81373843);College Graduate Student Innovative Research Fund of Jiangsu Province,China(No.CXZZ13-0613);Administration of Traditional Chinese Medicine Science and Technology Project of Jiangsu Province,China(No.LZ13006)

摘　　要：Objective： To study the effect of ligustrazine nanoparticles nano spray（LNNS） on transforming growth factor β（TGF-β）/Smad signal protein of rat peritoneal mesothelial cells（RPMC） induced by tumor necrosis factor α（TNF-α）, and the anti-adhesion mechanism of LNNS in the abdominal cavity. Methods： The primary culture and subculture of rat peritoneal mesothelial cells（RPMC） was processed by trypsin digestion method in vitro. The third generation was identified for experiment and divided into 5 groups： a blank group： RPMC without treatment; a control group： RPMC stimulated with TNF-α; RPMC treated by a low-dosage LNNS group（2.5 mg/L）; RPMC treated by a medium-dosage LNNS group（5 mg/L）; and RPMC treated by a high-dosage LNNS group（10 mg/L）. Reverse transcription-polymerase chain reaction was applied to test the expression of fibronectin, collagen Ⅰ（COL-Ⅰ）, TGF-β m RNA, and Western blot method to test the Smad protein 7 expression of RPMC. Results： Compared with the blank group, a significant elevation in fibronectin（FN）, COL-Ⅰ and TGF-β m RNA expression of RPMC were observed in the control group（P〈0.05）. Compared with the control group, LNNS suppressed the expressions of FN, COL-Ⅰ and TGF-β m RNA in a concentrationdependent manner（P〈0.05）. The expression of Smad7 protein of RPMC was down-regulated by TNF-α stimulation, and up-regulated with the increase of LNNS dose（P〈0.05）. Conclusions： TNF-α may induce changes in RPMC＇s viability, leading to peritoneal injury. LNNS could reverse the induction of fibrosis related cytokine FN, COL-Ⅰ and TGF-β, up-regulating the expression of Smad7 by TNF-α in RPMC, thus attenuate peritoneal injury by repairing mesothelial cells.Objective： To study the effect of ligustrazine nanoparticles nano spray（LNNS） on transforming growth factor β（TGF-β）/Smad signal protein of rat peritoneal mesothelial cells（RPMC） induced by tumor necrosis factor α（TNF-α）, and the anti-adhesion mechanism of LNNS in the abdominal cavity. Methods： The primary culture and subculture of rat peritoneal mesothelial cells（RPMC） was processed by trypsin digestion method in vitro. The third generation was identified for experiment and divided into 5 groups： a blank group： RPMC without treatment; a control group： RPMC stimulated with TNF-α; RPMC treated by a low-dosage LNNS group（2.5 mg/L）; RPMC treated by a medium-dosage LNNS group（5 mg/L）; and RPMC treated by a high-dosage LNNS group（10 mg/L）. Reverse transcription-polymerase chain reaction was applied to test the expression of fibronectin, collagen Ⅰ（COL-Ⅰ）, TGF-β m RNA, and Western blot method to test the Smad protein 7 expression of RPMC. Results： Compared with the blank group, a significant elevation in fibronectin（FN）, COL-Ⅰ and TGF-β m RNA expression of RPMC were observed in the control group（P〈0.05）. Compared with the control group, LNNS suppressed the expressions of FN, COL-Ⅰ and TGF-β m RNA in a concentrationdependent manner（P〈0.05）. The expression of Smad7 protein of RPMC was down-regulated by TNF-α stimulation, and up-regulated with the increase of LNNS dose（P〈0.05）. Conclusions： TNF-α may induce changes in RPMC＇s viability, leading to peritoneal injury. LNNS could reverse the induction of fibrosis related cytokine FN, COL-Ⅰ and TGF-β, up-regulating the expression of Smad7 by TNF-α in RPMC, thus attenuate peritoneal injury by repairing mesothelial cells.

关 键 词：peritoneal mesothelial cells ligustrazine experimental research transforming growth factor β/Smad 

分 类 号：R285.5[医药卫生—中药学]