骨髓活检标本乙二醇-甲基丙烯酸酯塑胶包埋切片免疫组织化学检测的研究  

Immunohistochemical detection with glycol methacrylate(GMA) plastic embedded section of bone marrow biopsy specimen

作　　者：浦杰[1] 石军[2] 陈真 姚永华 浦权[2,3] 

机构地区：[1]广西桂林医学院附属医院血液病科,广西桂林541001 [2]上海交通大学附属第六人民医院血液科,上海200233 [3]上海市东医院浦权血液病理诊断中心,上海200438

出　　处：《诊断学理论与实践》2016年第3期312-316,共5页Journal of Diagnostics Concepts & Practice

基　　金：桂教科研[2014](lx2014255)

摘　　要：目的:探讨丙酮固定骨髓标本乙二醇-甲基丙烯酸酯(glycol-methacrylate,GMA)塑胶包埋(塑包)切片内保留敏感造血分化抗原的新方法。方法:使用冷丙酮固定结合简易三步塑包体系,观察52例血液病患者骨髓GMA包埋切片内14种单克隆标志物的表达情况。结果:简易三步塑胶包埋体系不仅能提供良好的细胞形态,细胞抗原性也获较佳保存,并可在切片内进行c-myc原癌基因表达的检测。结论:建立了一种骨髓活检的简易塑包操作技术,既可保留良好的抗原性,也有助于观察免疫组织化学标志物相关的形态学细节。objective： To explore a new processing method that preserve the sensitive hematopoietic differentiation antigens in glycol-methacrylate（GMA） plastic embedded sections using acetone-fixed bone marrow biopsy sample.Methods： Expressions of 14 monoclonal markers in glycol-methacrylate embedded section prepared by cold acetonefixation with a simplified three-step glycol-methacrylate plastic embedding system were detected in 52 patients with hematological disorders. Results： The simplified three-step plastic embedding system could provide both excellent morphologic details and antigenic preservation, and the c-myc expression in bone marrow biopsy section could be detected.Conclusions： A simplified plastic embedding technique for bone marrow biopsy specimen is established, which allows excellent antigen preservation and high quality morphological details in relation with immunohistochemical markers.

关 键 词：丙酮固定 骨髓活检 塑胶包埋 免疫组织化学 

分 类 号：R446.8[医药卫生—诊断学]