脂联素基因多态性与早发心肌梗死的相关性研究  

Association between genetic variants in the adiponectin gene and premature myocardial infarction

作　　者：张闽[1] 彭瑜[2] 吕树铮[1] 

机构地区：[1]首都医科大学附属北京安贞医院心内科,100029 [2]兰州大学第一医院心内科

出　　处：《中华心血管病杂志》2016年第7期577-582,共6页Chinese Journal of Cardiology

基　　金：国家科技支撑计划（2011BAI11B05）

摘　　要：目的探讨中国汉族人群早发心肌梗死（PMI）与脂联素基因多态性的相关性。方法采用病例对照研究方法，于2013年3月至2015年1月在北京安贞医院连续人选716例患者，其中PMI患者306例为PMI组，412例冠状动脉造影阴性者为对照组。脂联素基因的17个单核苷酸多态性（SNP）为候选位点，应用质谱分析的方法对SNP进行分型。用）（。检验分析各SNP的等位基因及基因型频率在两组之间的差异，用logisitic回归方法分析各个SNP在各种遗传模型下与PMI的关联。酶联免疫分析方法检测血浆脂联素水平，分析脂联素的水平与SNP及PMI的相关性。结果病例组和对照组脂联素基因各SNP位点的基因型和等位基因的分布频率差异无统计学意义（P〉0．05）；用logisitic回归方法分别在相加、显性和隐性遗传3种遗传模型下进行SNP位点与PMI的关联分析，校正混杂因素后发现，在相加遗传模式下，rs2241766T〉G与PMI相关（OR=1．446，95％C／1．006～2．079，P=0．035）；在显性遗传模式下，rs12629945G〉A与PMI相关（OR=1．609，95％C／1．006～2．573，P=0．041）。PMI组脂联素水平低于对照组[（863．3±112．8）μg／L比（910．4±117．1）μg／L，P=0．042]，rs2241766T〉G纯合突变基因型（GG）患者脂联素水平低于杂合突变和野生基因型（GT＋TT）的患者[（859．4±98．1）μg／L比（908．9±113．1）μg／L，P=0．032]。结论脂联素基因rs2241766T〉G和rsl2629945G〉A的多态性与中国汉族人群PMI的易感性相关，rs2241766T〉G多态性引起脂联素水平下降，可增加PMI的发病风险。Objective To investigate the relationship between adiponectin gene polymorphisms and premature myocardial infarction （PMI）. Methods A total of 306 PMI patients of Beijing Anzhen Hospital were identified from March 2013 to January 2015 using the case-control method, 412 patients with normal coronary angiography results served as the control group. 17 single nucleotide polymorphisms （SNPs） in adiponectin gene were selected and genotyped using mass spectrometry. The Chi-squared test was used to compare allele and genotype frequencies between PMI and control groups. The plasma adiponectin concentrations were measured using enzyme-linked immunoassay, and the relationship was analyzed between adiponectin level and SNPs. Results There was no significant difference in allele frequencies and in genotype frequencies between PMI patients and controls （P 〉 0. 05 ）. Multivariate logistic regression models were used to estimate the correlation in additive, dominant and recessive genetic models, respectively. Analysis showed that rs2241766T 〉 G was significant associated with PMI risk in a addition model in Chinese Han population（ OR = 1. 446, 95% C1 1. 006 - 2. 079, P = 0. 035 ）, and rs12629945G 〉 A was significant association with PMI risk in a dominant model in Chinese Han population （ OR = 1. 609, 95% CI 1. 006 - 2. 573, P =0. 041 ）. Adiponeetin levels in PMI group were significantly lower than in control group （ （863.3 ± 112. 8 ） μg/L vs. （ 910. 4 ± 117. 1 ） μg/L, P = 0. 042 ）. Adiponectin levels were significantly lower in patients with the homozygous mutation genotype （GG） compared to patients with the heterozygote mutant andwild type genotypes （ GT ± TF） in adiponectin gene rs2241766 T 〉 G polymorphisms （ （859. 4 ± 98. 1 ） μg/L vs. （908.9 ± 113.1 ） μg/L, P = 0.032）. Conclusion The rs2241766T 〉 G and rs12629945G 〉 A polymorphisms in the adiponectin gene were associated with PMI in Chinese Han population. Rs2241766T 〉 G polymorphisms is linked with

关 键 词：心肌梗死 脂联素 多态性 单核苷酸 

分 类 号：R542.22[医药卫生—心血管疾病]