异氟醚麻醉对发育期大鼠海马Wnt3a表达的影响  

Effect of isoflurane anesthesia on Wnt3a expression in hippocampus of developing rats

作　　者：孙丽宁[1,2] 孙越[2] 齐军[2] 王雪芹[3] 李克忠[4] 

机构地区：[1]山东大学第二医院麻醉科,济南市250013 [2]青岛大学医学院附属烟台毓璜顶医院麻醉科 [3]临沂市人民医院麻醉科 [4]青岛大学医学院附属烟台毓璜顶医院麻醉科,264000

出　　处：《中华麻醉学杂志》2016年第5期551-553,共3页Chinese Journal of Anesthesiology

基　　金：高等学校博士点专项科研基金(20130131110043) Specialized Research Fund for the Doctoral Program of Higher Education

摘　　要：目的评价异氟醚麻醉对发育期大鼠海马Wnt3a表达的影响。方法取出生7d的雄性SD大鼠24只，体重125～155g，采用随机数字表法分为3组（n=8）：对照组（c组）、2％异氟醚吸入4h组（I。组）和2％异氟醚吸入6h组（I6组）。各组处理结束后6h时处死大鼠，取海马组织，采用RT—PCR法测定Wnt3amRNA的表达水平，采用Westernbolt法测定Wnt3a的表达水平。结果与C组比较，I4组和I6组海马组织Wnt3a及其mRNA表达上调（P〈0．05）；与I4组比较，I6组海马组织Wnt3a表达上调（P〈0．05），Wnt3amRNA表达差异无统计学意义（P〉0．05）。结论异氟醚麻醉对发育期大鼠产生神经毒性的机制可能与其上调海马组织Wnt3a的表达有关。Objective To evaluate the effect of isoflurane anesthesia on Wnt3a expression in the hippocampus of developing rats. Methods Twenty-four 7-day-old male Sprague-Dawley rats, weighing 125-155 g, were randomly divided into 3 groups （n = 8 each） using a random number table： control group （group C） , 4 h inhalation of 2% isoflurane group （group I4） , and 6 h inhalation of 2% isoflurane group （group 16） . The rats were sacrificed at 6 h after the end of treatment in each group, and the hippocampi were removed for determination of Wnt3a mRNA expression （ by real-time reverse transeriptase polymerase chain reaction） and Wnt3a expression （by Western blot）. Results Compared with group C, the expres- sion of Wnt3a and Wnt3a mRNA in hippocampi was significantly up-regulated in 14 and 16 groups （P〈 0.05）. Compared with group I4, the expression of Wnt3a in hippocampi was significantly up-regulated （P〈0.05） , and no significant change was found in the expression of Wnt3a mRNA in hippocampi in group 16 （P〉0. 05）. Conclusion The mechanism of isoflurane-indueed neurotoxicity is probably related to up- regulation of Wnt3a expression in the hippocampal tissues of developing rats.

关 键 词：异氟醚 婴儿 新生 WNT蛋白质类 海马 

分 类 号：R614[医药卫生—麻醉学]