瑞芬太尼诱发切口痛大鼠痛觉过敏时脊髓铁含量的变化  

Changes in spinal iron content during remifentanil-induced hyperalgesia in a rat model of incisional pain

作　　者：张麟临 李楠[1] 王春艳[1] 赵亓[1] 王海云[2] 于泳浩[1] 王国林[1] 

机构地区：[1]天津医科大学总医院麻醉科天津市麻醉学研究所,300052 [2]天津市第三中心医院麻醉科,300170

出　　处：《中华麻醉学杂志》2016年第5期581-583,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81371245,81301025,81400908）;天津市科技支撑计划重点项目（12ZCZDSY03000）

摘　　要：目的评价瑞芬太尼诱发切口痛大鼠痛觉过敏时脊髓铁含量的变化。方法清洁级健康成年雄性sD大鼠32只，体重240～260g，采用随机数字表法分为4组（n=8）：对照组（c组）尾静脉输注生理盐水0．1ml·kg-1·min-160min；切口痛组（I组）建立大鼠切口痛模型，尾静脉输注生理盐水0．1m-1·kg-1·min-1 60min；瑞芬太尼组（R组）尾静脉输注瑞芬太尼1．0uxg·kg-1·min。60min；切口痛＋瑞芬太尼组（I＋R组）尾静脉输注瑞芬太尼1．0I．zg·kg-1·min-1 60min，静脉输注10min时建立大鼠切口痛模型。分别于静脉输注前24h、输注结束后6、24和48h（T0-3）时测定机械缩足反应阈（MWT）和热缩足潜伏期（TwL）。最后1次测定痛阈后处死大鼠，取L4-6脊髓组织，采用普鲁士蓝铁染色和原子吸收分光光度计法测定脊髓铁含量。结果与C组比较，I组、R组和I＋R组T1-3时MWT降低，TWL缩短，脊髓铁含量升高（P〈0．05）；与I组和R组比较，I＋R组T1-3时MWT降低，TWL缩短，脊髓铁含量升高（P〈0．05）。结论瑞芬太尼诱发切口痛大鼠痛觉过敏的形成可能与脊髓铁含量升高有关。Objective To evaluate the changes in the spinal iron content during remifentanil-in- duced hyperalgesia in a rat model of incisional pain. Methods Thirty-two pathogen-free adult male Spra- gue-Dawley rats, weighing 240-260 g, were randomly divided into 4 groups （n = 8 each） using a random number table ： control group （ group C） , incisional pain group （ group I） , remifentanil group （ group R） , and incisional pain ＋ remifentanil group （group I＋R）. In I and I＋R groups, a 1 cm longitudinal incision was made in the plantar surface of left hindpaw in sevoflurane-anesthetized rats. Normal saline was infused at a rate of 0. 1 ml ~ kg-1 ~ rain-l for 60 min via the caudal vein in C and I groups. Remifentanil was infused at a rate of 1.0 p^g ~ kg-1 ~ rain-l for 60 rain via the caudal vein in R and I＋R groups. At 24 h before intra- venous infusion and 6, 24 and 48 h after the end of infusion （T0-3 ） , the mechanical paw withdrawal threshold （MWT） and thermal paw withdrawal latency （TWL） were measured. All the rats were sacrificed after the last measurement of pain thresholds, and the L4-6 segments of the spinal cord were removed for de- termination of iron content using the Perl＇s iron staining and atomic absorption spectrophotometer. Results Compared with group C, the MWT was significantly decreased, and the TWL was significantly shortened at T1-3, and the iron content was significantly increased in I, R and I＋R groups （P〈0.05）. Compared with I and R groups, the MWT was significantly decreased, and the TWL was significantly shortened at T1-3 , and the iron content was significantly increased in group I ＋ R （ P 〈 0. 05 ）. Conclusion The development of remifentanil-induced hyperalgesia is probably related to increased spinal iron content in a rat model of inci- sional pain.

关 键 词：哌啶类 痛觉过敏 铁 脊髓 

分 类 号：R971.2[医药卫生—药品]