浅低温对小鼠脑缺血再灌注时脑组织SENP3表达的影响  

Effect of mild hypothermia on expression of small ubiquitin-like modifier-specific proteases 3 in brain tissues during cerebral ischemia-reperfusion in mice

作　　者：汤莹莹[1] 陈怀龙[1] 刘孝洁[1] 刘冰[1] 王明山[1] 

机构地区：[1]青岛市市立医院麻醉科,266071

出　　处：《中华麻醉学杂志》2016年第6期682-684,共3页Chinese Journal of Anesthesiology

摘　　要：目的探讨浅低温对小鼠脑缺血再灌注时脑组织小泛素样修饰蛋白特异性蛋白酶3（SENP3）表达的影响。方法健康雄性C57／BL6小鼠96只，10～12周龄，体重22～30g，采用随机数字表法将其分为3组（n=32）：假手术组（S组）、脑缺血再灌注组（I／R组）和浅低温组（H组）。采用结扎双侧颈总动脉15rain再灌注的方法制备小鼠脑缺血再灌注损伤模型。H组于松开动脉夹即刻向小鼠全身喷洒酒精行体表降温，维持直肠温度32～34℃3h。分别于再灌注6、12、24和72h时，随机取8只小鼠，断头处死，取海马组织，光镜下观察病理学结果，TUNEL法检测神经元凋亡率，采用Western blot法测定海马SENP3表达水平。结果与S组比较，I／R组再灌注各时点海马CA1区神经元凋亡率升高，海马SENP3表达上调（P〈0．05）；与I／R组比较，H组再灌注各时点海马CA1区神经元凋亡率降低，海马SENP3表达下调（P〈0．05），病理学损伤减轻。结论浅低温减轻小鼠脑缺血再灌注损伤的机制与抑制脑组织SENP3的表达有关。Objective To investigate the effect of mild hypothermia on the expression of small ubiquitin-like modifier-specific proteases 3 （SENP3） in the brain tissues during cerebral ischemia-reperfusion （I/R） in mice. Methods Ninety-six male C57/BL6 mice, aged 10-12 weeks, weighing 22-30 g, were randomly divided into 3 groups （n = 32 each） using a random number table： sham operation group （group S） , group I/R, and mild hypothermia group （group H）. Cerebral I/R was produced by occlusion of bilateral common carotid arteries for 15 min followed by reperfusion. The surface cooling was started immediately after repeffusion, and the rectal temperature was maintained at 32-34℃ for 3 h. At 6, 12, 24 and 72 h of reperfusion, 8 mice were selected from each group and sacrificed. The hippocampi were re- moved for examination of the pathological changes （with light microscope） and for determination of cell apoptosis （using TUNEL） and expression of SENP3 （by Western blot）. The apoptosis rate was calculated. Results Compared with group S, the apoptosis rate in hippocampal CA1 region was significantly in- creased, and the expression of SENP3 was significantly up-regulated at each time point of reperfusion in group I/R （P〈0. 05）. Compared with group I/R, the apoptosis rate in hippocampal CA1 region was significantly decreased, and the expression of SENP3 was significantly down-regulated at each time point of reperfusion （P〈0. 05） , and the pathological changes were significantly reduced in group H. Conclusion The mechanism by which mild hypothermia reduces cerebral I/R injury is associated with inhibition of SENP3 expression in the brain tissues of mice.

关 键 词：低温 人工 半胱氨酸内肽酶类 再灌注损伤 脑 

分 类 号：R614[医药卫生—麻醉学]