丙泊酚对大鼠胶质瘤细胞侵袭和迁移能力的影响及ADAR2-AMPA受体GluR2通路在其中的作用  

Effects of propofol on invasion and migration of glioma cells in rats and the role of ADAR2-AMPA receptor GInR2 pathway

作　　者：王欣悦[1] 王海云[1] 王国林[2] 杨卓[3] 张涛[3] 

机构地区：[1]天津医科大学三中心临床学院天津市第三中心医院麻醉科,300170 [2]天津医科大学总医院,300052 [3]南开大学药物化学生物国家重点实验室,天津市300071

出　　处：《中华麻醉学杂志》2016年第6期712-715,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81071059,81100984,81571054）;天津市应用基础与前沿技术研究计划（一般项目）（15JCYBJc25600）

摘　　要：目的评价丙泊酚对大鼠胶质瘤细胞侵袭和迁移能力的影响及腺苷脱氨酶（ADAR2）-α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体GluR2通路在其中的作用。方法传代培养大鼠C6胶质瘤细胞，采用随机数字表法分为4组（n=24）：对照组（C组）、丙泊酚组（P组）、阴性siRNA转染＋丙泊酚组（NP组）和ADAR2-siRNA转染＋丙泊酚组（AP组）。C组正常培养；NP组和AP组分别将阴性siRNA或ADAR2-siRNA转染至细胞内，48h后处理同P组；P组加入丙泊酚，终浓度1．2μg／ml，孵育6h后换为正常培养液，继续培养18h。采用MTT比色分析法检测细胞活力，Transwell侵袭实验测定侵袭细胞数，细胞划痕实验测定迁移率，Western blot法检测胞核ADAR2和胞膜GluR2的表达。结果与C组比较，P组和NP组细胞活力、侵袭细胞数和迁移率降低，胞核A—DAR2及胞膜GluR2表达上调（P〈0．05）；与P组比较，AP组细胞活力、侵袭细胞数和迁移率升高，胞核ADAR2及胞膜GluR2表达下调（P〈0．05）；与NP组比较，AP组细胞活力、侵袭细胞数和迁移率升高，胞核ADAR2及胞膜GluR2表达下调（P〈0．05）。结论丙泊酚可抑制大鼠胶质瘤细胞的侵袭和迁移能力，其机制与激活ADAR2-AMPA受体GluR2通路有关。Objective To evaluate the effects of propofol on the invasion and migration of glioma cells in the rats and the role of adenosine deaminase acting on RNA 2 （ ADAR2）-α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid （AMPA） receptor subunit glutamate 2 （GluR2） pathway. Methods C6 glioma cells were subculturcd and randomly divided into 4 groups （n= 24 each） using a random number table： control group （group C） ; propofol group （group P） ; negative siRNA transfection ＋ propofol group （group NP） ; ADAR2-siRNA transfection ＋ propofol group （ group AP）. The ceils were cultured in the common culture medium in group C. In NP and AP groups, negative siRNA and ADAR2-siRNA were transfected into the cells, respectively, and 48 h later the other procedures were similar to those previously described in group P. Propofol with the final concentration of 1.2μg/ml was added, the cells were cultured for 6 h and then were cultured in the common culture medium for another 18 h in group P. The cells were selected to detect the cell viability by MTT colorimetric assay. The invasion of cells was determined by Transwell invasion assay, and the invaded cells were counted. The migration of cells was determined by cell scratch test, and cell migration rates were calculated. The expression of ADAR2 in the nucleus of cells and GluR2 in the cytomembrane was detected by Western blot. Results Compared with group C, the cell viability, the number of invaded cells and cell migration rates were significantly decreased, and the expression of ADAR2 in the nucleus of cells and GluR2 in the cytomembrane was significantly up-regulated in P and NP groups （P〈0.05）. Compared with group P, the cell viability, the number of invaded cells and cell migration rates were significantly increased, and the expression of ADAR2 in the nucleus of cells and GluR2 in the cytomembrane was significantly down-regulated in group AP （P〈0.05）. Compared with group NP, the cell viability, the number of invaded cells and cell

关 键 词：二异丙酚 神经胶质瘤 细胞系 肿瘤 肿瘤侵润 肿瘤转移 

分 类 号：R614[医药卫生—麻醉学] R739.41[医药卫生—外科学]