异丙酚对术前睡眠剥夺诱发大鼠术后认知功能障碍的影响  

Effect of propofol on postoperative cognitive dysfunction induced by preoperative sleep deprivation in rats

作　　者：王红柏[1] 王卫娜[1] 李平[1] 张华[1] 

机构地区：[1]天津市天津医院麻醉科,300211

出　　处：《中华麻醉学杂志》2016年第6期732-735,共4页Chinese Journal of Anesthesiology

摘　　要：目的评价异丙酚对术前睡眠剥夺诱发大鼠术后认知功能障碍的影响。方法雄性成年SD大鼠126只，体重200～250g，采用随机数字表法分为3组（n=42）：对照组（C组）、睡眠剥夺组（SD组）和睡眠剥夺＋异丙酚组（SD＋P组）。SD组和SD＋P组通过跑步机经历持续96h的睡眠剥夺；然后3组均行右侧股骨干骨折髓内钉内固定术。SD＋P组术毕即刻经健侧股静脉输注异丙酚20mg·kg^-1·h^-1，持续输注2h；C组和sD组术毕即刻经健侧股静脉输注生理盐水2ml·kg^-1·h^-1，持续输注2h。术后1-5d，行Morris水迷宫实验测试认知功能，记录逃避潜伏期和原平台位置停留时间。分别于术后1、3和7d时处死大鼠，取海马组织，行尼氏染色，行CA1区神经元计数；行高尔基染色，测定CA1区神经元树突棘密度。结果与C组比较，SD组逃避潜伏期延长，原平台位置停留时间缩短，术后各时点海马CA1区神经元计数和树突棘密度降低（P〈0．05），SD＋P组上述各指标差异无统计学意义（P〉0．05）；与SD组比较，SD＋P组逃避潜伏期缩短，原平台位置停留时间延长，术后各时点海马CA1区神经元计数和树突棘密度升高（P〈0．05）。结论异丙酚可减轻术前睡眠剥夺诱发大鼠术后认知功能障碍。Objective To evaluate the effect of propofol on postoperative cognitive dysfunction induced by preoperative sleep deprivation in the rats. Methods A total of 126 healthy adult male Sprague- Dawley rats, weighing 200- 250 g, were randomly divided into 3 groups （ n = 42 each） using a random number table： control group （group C ）, sleep deprivation group （group SD）, and sleep deprivation ＋ propofol group （group SD＋P）. The rats were kept for 96 h on a treadmill that moved for 3 s on/12 s off in SD and SD＋P groups. Intramedullary nailing for right femoral shaft fractures was performed in the 3 groups. Propofol 20 mg·kg^-1·h^-1 was infused for 2 h via the contralatcral femoral vein starting from the end of op- eration in group SD＋P, while the equal volume of normal saline was infused for 2 h starting from the end of operation in C and SD groups. The animals underwent Morris water maze test at 1-5 days after operation to assess the cognitive function, and the escape latency and time of staying at the original platform position were recorded. At 1, 3 and 7 days after operation, bippocampal tissues were obtained, nissl staining was performed to determine neuron count in hippocampal CA1 area, and Golgi staining was performed to meas- ure the density of dendritic spine in hippocampal CA1 area. Results Compared with group C, the escape latency was significantly prolonged, the time of staying at the original platform position was significantly shortened, and the neuron count and density of dendritic spine in hippocampal CA1 area were significantly decreased at each time point after operation in group SD （P〈0.05） , and no significant change was found in each index mentioned above in group SD＋P （P〉0. 05）. Compared with group SD, the escape latency was significantly shortened, the time of staying at the original platform position was significantly prolonged, and the neuron count and density of dendritic spine in hippocampal CA1 area were significantly increased at each time point after o

关 键 词：二异丙酚 睡眠剥夺 认知障碍 

分 类 号：R614[医药卫生—麻醉学]