富氢液对失血性休克复苏大鼠肝损伤的影响  

Effect of hydrogen-rich saline on liver injury in a rat model of hemorrhagic shock and resuscitation

作　　者：党杨杰 孟祥忠[1] 徐浩[2] 每晓鹏[1] 邓斌[1] 赵朝华[2] 米亚静[1] 范莹盈[3] 徐礼鲜[1] 

机构地区：[1]军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔生物工程技术研究中心第四军医大学口腔医学院麻醉科,西安市710049 [2]西安医学院基础医学研究所,710021 [3]西安交通大学生命科学与技术学院线粒体生物医学研究所/生物医学信息工程教育部重点实验室,710049

出　　处：《中华麻醉学杂志》2016年第6期748-750,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81500063）;陕西省自然科学基础研究计划（2016JQ8049）;西安医学院科研基金（2015DOC12）

摘　　要：目的评价富氢液对失血性休克复苏大鼠肝损伤的影响。方法清洁级健康雄性SD大鼠48只，6～8周龄，体重150～250g。采用随机数字表法分为3组（n=16）：假手术组（Sham组）、失血性休克复苏组（HR组）和富氢液组（HRS组）。采用改良Wigger法建立大鼠重度失血性休克模型后，分别用乳酸林格液和富氢液进行复苏，于复苏后6h时采集股动脉血，处死大鼠取肝脏，采用自动生化分析仪测定血清ALT和AST的浓度，采用试剂盒检测肝组织T-SOD、MDA、TNF-α和IL-6水平，光镜下观察肝组织病理学结果。结果与Sham组比较，HR组和HRS组复苏后6h时血清ALT和AST、肝组织IL-6、TNF-α、T—SOD和MDA水平升高（P〈0．01）；与HR组比较，HRS组血清ALT和AST、肝组织IL-6、TNF-α和MDA水平降低，T—SOD水平升高（P〈0．01）。HRS组肝组织病理学损伤较HR组减轻。结论富氢液可减轻失血性休克复苏大鼠的肝损伤。Objective To evaluate the effects of hydrogen-rich saline on liver injury in a rat model of hemorrhagic shock and resuscitation. Methods Forty-eight pathogen-free male Sprague-Dawley rats, aged 6-8 weeks, weighing 150-250 g, were randomly divided into 3 groups （n= 16 each） using a random number table： sham operation group （group Sham）, hemorrhagic shock and resuscitation group （group HR）, and hydrogen-rich saline group （group HRS）. Severe hemorrhagic shock was induced according to the method described by Wiggers. Lactated Ringer＇s solution and hydrogen-rich saline were given for resuscitation in HR and HRS groups, respectively. At 6 h after resuscitation, blood samples were collected from the femoral artery for determination of serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） concentrations by automatic biochemical analyzer. The rats were then sacrificed, and liver specimens were obtained for examination of histopathological changes （using light microscope） and for determination of total superoxide dismutase （T-SOD） activity, malondialdehyde （MDA） content, and tumor necrosis factor-alpha （TNF-α） and interleukin-6 （IL-6） concentrations （ using the corresponding kits）. Results Compared with group Sham, the levels of ALT and AST in serum and IL-6, TNF-α, T-SOD and MDA in liver tissues were significantly increased in HR and HRS groups （P〈0. 01 ）. Compared with group HR, the levels of ALT and AST in serum and IL-6, TNF-α, and MDA in liver tissues were significantly decreased, and the T-SOD level was significantly increased in group HRS （P〈0. 01 ）. The histopathological changes of livers were significantly attenuated in group HRS as compared with group HR. Conclusion Hydrogen-rich saline can reduce liver injury in a rat model of hemorrhagic shock and resuscitation.

关 键 词：氢 休克 出血性 肝 再灌注损伤 

分 类 号：R459.7[医药卫生—急诊医学]