耕种模式对三江平原黑土细菌多样性的影响  

Effects of Cultivation Mode on Bacterial Diversity of Black Soil in Sanjiang Plain

作　　者：汪城墙[1] 黄峤璟 吕刚 胡文豪[2] 朱瑞敏[1] 丁延芹[1] 杜秉海[1] 

机构地区：[1]山东农业大学生命科学学院、山东省农业微生物重点实验室,山东泰安271018 [2]山东农业大学农学院,山东泰安271018 [3]临沂市河东区农业局,山东临沂276000

出　　处：《山东农业科学》2016年第7期76-81,共6页Shandong Agricultural Sciences

基　　金：中国博士后基金项目(2015M582121);山东农业大学学生发展资助项目(2015);山东农业大学博士后基金项目(76474);山东农业大学青年科技创新基金项目(24070);山东省自然科学基金项目(ZR2014CL003);山东省科技重大专项(2015ZDXX0502B02)

摘　　要：本试验使用Illumina Mi Seq测序平台,选取我国东北三江平原地区的大田黑土、菜园黑土和森林黑土3种样本,对不同耕种黑土表层细菌群落结构进行分析。结果显示:大田土、菜园土和森林土的微生物群落存在明显差异。大田土的群落丰富度明显低于菜园土和森林土,后两者相似度较高。大田土的OTU数比菜园土和森林土分别少13.3%和7.6%。菜园土的微生物群落组成种类最为丰富,森林土中硝化螺旋菌门含量最高,大田土微生物群落含有的外源性物质降解相关基因相对丰度较高。可见,三江平原大田黑土的退化和土壤微生物群落的改变有关,调节土壤微生物群落结构将有助于东北大田黑土的修复。The bacterial community structure of field,vegetable garden and forest black soil in Sanjiang Plain in the northeast of China were analyzed using Illumina Mi Seq sequencing platform under different cultivation modes. The results showed that the microbial community of field,vegetable garden and forest soil existed obvious differences. The community richness of field soil was significantly lower than that of vegetable garden and forest soil,and the latter two were similar. Compared with vegetable garden and forest soil,the OTU number of field soil reduced by 13. 3% and 7. 6% respectively. The microbial community composition of vegetable garden soil was the most abundant. The bacteria of Nitrospirae in forest soil held the highest level. The microbial community of field soil contained more abundant degradation genes of exogenous substances. Obviously,the black soil degradation in Sanjiang Plain was related to the changes of soil microbial community,and regulating the structure of soil microbial community was beneficial to the repair of field black soil.

关 键 词：细菌群落多样性 高通量测序 黑土地 耕种模式 

分 类 号：S154.36[农业科学—土壤学]