HLA-B27^+的强直性脊柱炎与非强直性脊柱炎患者外周血T淋巴细胞亚群及免疫炎症指标分析  

Analysis of T cell sub immune inflammation indices in peripheral blood of HLA- B27^+ ankylosing spondylitis and non- ankylosing spondylitis patients

作　　者：尤金彪[1] 毛向红[1] 董浙清[1] 邵笑[1] 祝骥[1] 方揆 

机构地区：[1]浙江中医药大学附属第三医院检验科,浙江杭州310000

出　　处：《中国卫生检验杂志》2016年第14期2039-2041,2044,共4页Chinese Journal of Health Laboratory Technology

基　　金：浙江中医药大学附属第三医院医药卫生科研计划(ZA-13CA03)

摘　　要：目的探讨HLA-B27^+的不同研究对象T淋巴细胞亚群以及免疫炎症指标的相关性与差异性,从而辅助AS和非AS疾病的早期鉴别。方法本研究以HLA-B27^+的AS患者、HLA-B27^+的非AS患者、HLA-B27^+的未发病者为研究对象,以HLA-B27-的健康人群为对照组。通过流式细胞术观察HLA-B27 MFI和T淋巴细胞亚群分布、速率散射比浊法检测ASO、RF、ESR、CRP指标。结果 HLA-B27~^+AS患者、非AS患者与未发病者的HLA-B27 MFI差异无统计学意义(P>0.05),ASO和RF阳性率差异无统计学意义(P>0.05)。HLA-B27^+AS患者、HLA-B27^+非AS患者的ESR、CRP、CD4^+和CD8^+T细胞百分率、CD4^+T/CD8^+T比值与健康人群组相比,差异均有统计学意义(P<0.05)。HLA-B27^+AS患者的ESR、CRP水平高于HLA-B27^+非AS患者,差异有统计学意义(P<0.05)。HLA-B27^+AS患者CD8^+T细胞百分率高于HLA-B27^+非AS患者,CD4^+T/CD8^+T比值低于HLA-B27^+非AS患者,差异均有统计学意义(P<0.05)。结论 HLA-B27^+AS患者与HLA-B27^+非AS患者早期可以通过ESR、CRP、CD8^+T细胞百分率、CD4^+T/CD8^+T比值加以鉴别。Objective To study the relevance and difference of T lymphocytes subsets and immune inflammatory indices in dif- ferent research objects of HLA - B27 ＋ , so as to help the early identification of ankylosing spondylitis（AS） and non - ankylosing spondylitis（AS）. Methods In this study, HLA -B27＋ AS patients, HLA -B27＋ non -AS patients, HLA -B27＋ patients without the disease were selected as the research objects, with HLA -B27- healthy population as the control group. HLA -B27 mean fluorescence intensity （MFI） and T lymphocytes subsets distribution were observed by flow cytometry, and ASO, RF, ESR, CRP indices were detected by rate scattering turbidimetry method. Results The differences of HLA - B27 MFI in HLA - B27 ＋ AS patients, non -AS patients, patients without the disease the differences had no statistical significance（ P 〉 0.05 ） ; and the differences of ASO and RF positive rates had no statistical significance （ P 〉 0.05 ）. The differences of ESR, CRP, the percent- age of CD4＋and CD8＋ T cells, the ratio of CD4 ＋ T/CD8＋ T between the HLA - B27＋ AS patients, HLA - B27＋ non - AS pa- tients and the healthy population groups had statistical significance（ P 〈 0.05 ）. The levels of ESR and CRP in HLA- B27＋ AS patients were higher than that of the HLA - B27＋ non - AS patients, and the differences were statistically significant （ P 〈 0.05 ）. The percentage of CD8 ＋ T cells in HLA - B27＋ AS patients was higher than that of HLA - B27＋ non - AS patients, the ratio of CD4 ＋ T/CD8＋ T was lower than that of HLA - B27＋ non - AS patients, and the differences were statistically significant （P 〈 0.05）. Conclusion HLA- B27α AS patients and HLA -B27＋ non- AS patients can be distinguished using the ESR, CRP, the percentage of CD8＋ T cells and the ratio of CD4 ＋ T/CD8 ＋ T.

关 键 词：强直性脊柱炎 HLA-B27 T淋巴细胞亚群 炎症指标 

分 类 号：R593.23[医药卫生—内科学]