GABA对热应激雏鸡小肠黏膜中IL-IL-7和sIgA含量的影响  

Effect of Dietary GABA on Content Changes of sIgA and IL-7 of Intestinal Mucosa in Heat-stressed Chicks

作　　者：梁臣[1] 汪威[1] 周永蔚 姜迎娅[1] 陈忠[1] 

机构地区：[1]海南师范大学热带动植物生态学教育部重点实验室,海南海口571158

出　　处：《中国家禽》2016年第13期22-25,共4页China Poultry

基　　金：国家自然科学基金项目(31260555、31560680)

摘　　要：为探究Y-氨基丁酸（GABA）对热应激雏鸡小肠黏膜中IL-7和slgA含量的影响，选择体重相近的1日龄文昌雏公鸡，随机分为对照（CK）组、热应激（HS）组、50mg／kgGABA（G50＋HS）组和100mg／kgGABA（G100＋HS）组，每组36只，每天13：00～15：00将HS组、（G50＋HS）组和（G100＋HS）组置于（40＋0．5）℃人工气候箱中进行热处理，利用ELISA法测定不同周龄雏鸡小肠黏膜液中IL-7和sIgA含量。结果：HS组的十二指肠、空肠及回肠中1L-7和sIn含量显著低于CK组（P〈0．05）；（G50＋HS）组和（G100＋HS）组十二指肠、空肠及回肠中IL-7和sIgA含量显著高于HS组（P〈0．05）；（G100＋HS）组部分肠段中的IL-7和slgA含量与（G50＋HS）组比较有显著差异（P〈0．05）。研究结果表明GABA对热应激造成的肠道损伤具有一定修复作用，能够有效提高热应激状态下雏鸡小肠黏膜的免疫功能，但其保护作用并不与浓度呈正比。This study was aimed to investigate the effect of dietary GABA on content changes of slgA and IL-7 of intestinal mucosa of chicks under heat stress. One-day-age male Wenchang chicks were randomly divided into CK group,HS group and 50 mg/kg GABA （G50＋HS）group and 100 mg/kg GABA （G100＋HS）group. Chicks from HS, （G50＋HS） and （G100＋HS）groups were subjected to heat stress treatment at （40＋0.5）~C for 2h every day （13：00 to 15：00）. ELISA was used to measure IL-7 and sIgA contents of small intestinal fluid in different-age chicken. The results showed that IL-7 and sIgA of duodenum, jejunum and ileum in HS group were significantly lower than that in CK group （P〈0.05）. The IL-7 and sIgA of duodenum,jejunum and ileum in （G50＋HS） and （G100＋HS） groups were significantly higher than that in HS group （P〈0.05）. There was a significant difference in IL-7 and slgA of part of intestinal between （G100＋HS） group and （G50＋HS） group （P〈0.05）. It was concluded that GABA alleviated thenegative effects on development of chick intestinal mucosa, improved the innnune function of intestinal mucosa and played a protective role,but its protective effect was not directly proportional to the concentration.

关 键 词：热应激 IL-7 sIgA GABA 小肠黏膜 雏鸡 

分 类 号：S831.5[农业科学—畜牧学]