续断属植物SRAP标记的遗传亲缘关系分析  

Genetic Relations Analysis in Dipsacus Plant Based on SRAP

作　　者：李晓玲[1,2] 程岁寒[1,2] 杨进[1] 

机构地区：[1]三峡大学生物与制药学院,湖北宜昌443002 [2]三峡库区生态环境教育部工程研究中心(三峡大学),湖北宜昌443002

出　　处：《西南农业学报》2016年第7期1554-1558,共5页Southwest China Journal of Agricultural Sciences

基　　金：湖北省重点实验室开放基金“天然产物研究与利用”(2008NP09)

摘　　要：本文采用10对SRAP引物，对10份续断属植物样本进行遗传亲缘关系分析。结果表明，10对引物共扩出566条谱带，平均每对引物扩增出56．6条带，片段大小为150—2000bp，其中多态性条带为362条，平均每对引物扩增的多态性条带为36．2条，平均多态性条带百分率为64％，结果表明续断属植物具有丰富的遗传变异。UPGMA聚类分析表明10份续断属植物分为2个大类和4个亚类，大头续断和深紫续断有一定的亲缘关系，与其它续断属植物问遗传差异较大；日本续断、恩施续断、大理续断和川续断遗传亲缘关系较近，其中大理续断和川续断聚为一个小类群，支持大理续断并入川续断。10 SRAP primer pairs were used to analyze the genetic relationship of 10 Dipsacu.s materials. The results showed that 10 primer pairs produced 566 bands with average 56.6 bands by each primer, of which 362 bands were polymorphisms with average 36.2 bands, accounting for 64 %, and the length were 150 -2000 bp, which indicated that Dipsacus plant had abundant genetic variation. According to the UPGMA cluster analysis, the ten pieces of Dipsacus could be gathered into 2 big groups or 4 subgroups, DT and SZ were distance from any other accessions, and the genetic relationship between them was very close; DL, ES, RB, C （J） and C （D） had close relationships between them, and C（J） and C（D） belonged to the same subgroup, which supported that D. daliensis T. M. Ai. Belonged to D. asperoides C. Y. Cheng et T. M. Ai.

关 键 词：续断属植物 SRAP 遗传亲缘关系 

分 类 号：S567.23[农业科学—中草药栽培]