四川地区腹泻仔猪群猪圆环病毒Ⅱ型分子流行病学调查  

Molecular epidemiology of porcine circovirus type Ⅱ in diarrhea piglets in Sichuan region

作　　者：赵军[1] 周桐枫 刘鹏娟[1] 黄剑波[1] 姜子义[1] 朱玲[1,2] 徐志文[1,2] 

机构地区：[1]四川农业大学动物医学院,四川成都611130 [2]四川农业大学动物疫病与人类健康四川省重点实验室,四川成都611130

出　　处：《中国预防兽医学报》2016年第7期546-549,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：四川省科技支撑计划(2014NZ0043);科技部国际科技合作项目(2014DFA31260)

摘　　要：为了解四川地区腹泻仔猪群中猪圆环病毒Ⅱ型(PCV2)的感染情况,本实验采用PCR方法对病料样品进行PCV2检测,并对分离的病毒DNA进行测序及生物信息学分析。结果显示PCV2感染率为71.6%(312/436),阳性结果中肠系膜淋巴结检出率为100%(312/312)。将扩增并测序的19条PCV2序列与Gene Bank中23株参考序列比较分析,构建了系统进化树,结果表明四川省腹泻仔猪群感染PCV2基因型主要为PCV2b、PCV2d基因型。针对PCV2高频突变ORF2基因编码的Cap蛋白氨基酸序列进行生物信息学分析,结果显示主要抗原表位氨基酸位点存在替换,进而引起抗原漂移,这可能促使PCV2免疫原性发生改变。本研究为四川地区PCV2的防制奠定了基础。To investigate the molecular epidemiology of porcine circovirus type Ⅱ （PCV2） in Sichuan region, a total of 436 clinical samples were collected from diarrhea piglets on different pig farms in 13 areas, and subjected to the PCV2 detection by PCR. The results showed PCV2 positive rate was 71.6% （312/436）. In addition, a phylogenetic tree was constructed based on the complete genomic sequences of 19 isolates and other 23 PCV2 complete genomic sequences from the reference strains, and the genotypes of the prevalent PCV2 in Sichuan region were PCV2b and PCV2d. Moreover, the amino acids substitutions were found in the major epitope region of capsid protein, which might cause the antigenic drift and altere the immunogenicity of PCV2.

关 键 词：腹泻仔猪 圆环病毒Ⅱ型 CAP蛋白 分子流行病学 

分 类 号：S852.65[农业科学—基础兽医学]