新疆伊犁地区马疱疹病毒1型的分离与鉴定  

Isolation and identification of equine herpesvirus 1 in Xinjiang

作　　者：杨永龙[1] 刘建华[1] 宋焕堂 李静[1] 卢亚宾 胡月[1] 范斌[1] 况玲[1] 冉多良[1] 

机构地区：[1]新疆农业大学动物医学学院,新疆乌鲁木齐830052

出　　处：《中国预防兽医学报》2016年第7期550-553,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家科技支撑计划项目(2012BAD46B00)

摘　　要：为鉴定新疆伊犁地区某马场疑似感染马鼻肺炎感染的病原,本实验采集流产胎儿肺组织,通过细胞培养、PCR鉴定、毒价测定、病毒中和试验、透射电镜观察以及病毒理化特性测定等方法进行了病毒的分离与鉴定。结果表明:MDBK细胞接种病毒后出现典型的细胞圆缩、细胞融合、细胞脱落和聚堆成葡萄串状等病变特征;毒价测定为TCID50106.2/m L;选择EHV-1的g B基因经PCR扩增,得到622 bp的特异性DNA片段;经理化特性测定该病毒不耐酸、不耐热、对紫外/氯仿/乙醚处理敏感;电镜观察可见圆形、有囊膜、直径大约为130 nm的病毒颗粒。综上所述,经分离与鉴定获得的病毒株生物学性质符合马疱疹病毒1型,并命名为EHV-1-XJ2015株。本研究在新疆伊犁分离到的马疱疹病毒1型属首次报道,为我国马鼻肺炎的防治奠定了基础。In this study, a virus was isolated from the lung tissue sample of an aborted fetuse suspected for equine rhinopneumonitis on a horse farm in Yili area of Xingjiang region. The identification results showed that the virus isolate was able to cause typical cytopathogenic effects in MDBK cells, and the virus titer reached 106.2 TCIDso/mL. In addition, a specific 622 bp fragment for gB gene of equine herpesvirus 1 （EHV-I） was amplified by PCR. The virus isolate was sensitive to acid, heat, and ultraviolet/chloroform/ether treatment. Electron microscopy examination showed round virus particles with capsule membrane, and its diameter was about 130 nm. In conclusion, these data indicated that the virus isolate is EHV-1, named XJ2015 strains. However, this is the first report that EHV-1 from Yili area, which laid the foundation for equine rhinopneumonitis prevention in this area.

关 键 词：伊犁马 马疱疹病毒1型 分离鉴定 

分 类 号：S852.65[农业科学—基础兽医学]