电针小海与下巨虚穴对DU模型大鼠ChAT及α7 nAchR的影响  

Effect of Electroacupuncture at Points Xiaohai and Xiajuxu on ChAT and α7 nAchR in a Rat Model of DU

作　　者：邓石峰 许明[1] 倪伟[1] 张雨辰[2] 杨璐佳 张泓[1] 

机构地区：[1]湖南中医药大学,长沙410007 [2]湖南中医药大学第二附属医院,长沙410208

出　　处：《上海针灸杂志》2016年第7期876-880,共5页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金项目(81173327/H2718)

摘　　要：目的观察电针十二指肠溃疡(duodenal ulcer,DU)模型大鼠小海、下巨虚对大鼠血清肿瘤坏死因子-a(tumour necrosis factor-a,TNF-a)、血清乙酰胆碱转移酶(choline acetyltransferase,Ch AT)及十二指肠组织烟碱型乙酰胆碱受体α7(neuronal acetylcholine receptorsα7,α7 nAchR)等表达的影响,初步探讨"合治内府"中"合"穴的相对特异性。方法将40只健康SD大鼠随机分为空白组(A组)、模型组(B组)、小海组(C组)和下巨虚组(D组),每组10只。于大鼠右臀部皮下部注射10%盐酸半胱胺建立大鼠DU模型,造模成功后,C组电针小海穴,D组电针下巨虚穴。肉眼观察各组大鼠十二指肠组织溃疡并评分,采用双抗体夹心酶联免疫吸附测定(ELISA)法检测大鼠血清中TNF-a表达,用可见紫外分光光度计进行比色,测量大鼠血清中Ch AT的表达,蛋白质印迹法(western blot)检测大鼠十二指肠组织中α7 nAchR的表达。结果造模后,B组、C组和D组大鼠十二指肠溃疡评分较A组显著增高(均P<0.01),C组、D组评分显著低于B组(均P<0.01),且D组显著低于C组(P<0.01)。B组TNF-a表达较A组显著增高(P<0.01),C组、D组TNF-a显著低于B组(均P<0.01),且D组显著低于C组(P<0.01)。C组Ch AT表达较B组有升高趋势但无统计学意义(P>0.05),D组Ch AT表达明显高于B组(P<0.01),且高于C组(P<0.05)。与B组相比,C组和D组α7 nAchR表达显著增高(均P<0.01)。各组Ch AT与α7 nAchR表达呈显著的正相关(r=0.444,P=0.007)。结论电针小海、下巨虚均能降低DU大鼠血清中十二指肠溃疡评分及TNF-a的表达,升高血清中Ch AT与十二指肠组织中α7 nAchR的表达,说明电针对十二指肠溃疡产生治疗作用有可能是通过调节TNF-a等来实现的,其机制有可能是通过激活胆碱能抗炎通路产生抗炎作用来完成的。D组效果优于C组,提示下巨虚存在相对特异性。Objective To investigate the effect of electroacupuncture at points Xiaohai and Xiajuxu on the expressions of tumour necrosis factor-a（TNF-a） and choline acetyltransferase（Ch AT） in serum and nicotinic acetylcholine receptor α7（α7 nAchR） in duodenal tissues in a rat model of duodenal ulcer（DU） and preliminarily explore the relative specificity of He-Sea point in ＂treating visceral diseases with He-Sea point＂. Methods Forty healthy SD rats were randomized into blank（A）, model（B）, Xiaohai（C） and Xiajuxu（D） groups, 10 rats each. A rat model of DU was made by subcutaneous injection of 10% cysteamine hydrochloride at the right buttock. After successful model making, group C was given electroacupuncture at point Xiaohai and group D, at point Xiajuxu. Duodenal tissue ulcer was macroscopically observed and scored in every group of rats. Rat serum expression of TNF-a was determined by double antibody sandwich enzyme-linked immunosorbent assay（ELISA）; rat serum expression of Ch AT, by ultraviolet spectrophotometry colorimetry; rat duodenal expression of a7 nAchR, by Western blot. Results After model making, the duodenal ulcer score was significantly higher in groups B, C and D than in group A（all P〈0.01） and significantly lower in groups C and D than in group B（both P〈0.01） and in group D than in group C（P〈0.01）. TNF-a expression was significantly higher in group B than in group A（P〈0.01） and significantly lower in groups C and D than in group B（both P〈0.01） and in group D than in group C（P〈0.01）. Ch AT expression tended to increase in group C compared with group B but there was no statistically significant difference（P〈0.05） and was significantly higher in group D than in group B（P〈0.01） or C（P〈0.05）. a7 nAchR expression was significantly higher in groups C and D than in group B（both P〈0.01）. There was a positive correlation between Ch AT and a7 nAchR expressions in every group（r=0.444, P=0.007）. Conclusions Electroac

关 键 词：电针 胆碱能抗炎通路 穴 小海 穴 下巨虚 十二指肠溃疡 血清肿瘤坏死因子-Α 血清乙酰胆碱转移酶 十二指肠组织烟碱型乙酰胆碱受体α7 大鼠 

分 类 号：R2-03[医药卫生—中医学]