骨髓间充质干细胞联合超短波对大鼠脊髓损伤后早期GFAP和ED-1的影响  

Effects of Bone Marrow Stromal Cells Transplantation Combined with Ultrashort Wave Therapy on the Expression of GFAP and ED-1 after Spinal Cord Injury in Rats

作　　者：孙师[1] 万峪岑 赵利娜[1] 尹艳梅[1] 陆宇[1] 冯智萍 周禹鑫[1] 张志强[1] 张立新[1] 

机构地区：[1]中国医科大学附属盛京医院康复科,沈阳110022

出　　处：《中国医科大学学报》2016年第8期678-683,共6页Journal of China Medical University

基　　金：国家自然科学基金青年科学基金(81101462)

摘　　要：目的探讨骨髓间充质干细胞(BMSCs)联合超短波治疗对大鼠脊髓损伤(SCI)后4周功能恢复、胶质纤维酸性蛋白(GFAP)和ED-1表达的影响。方法 30只雌性SD大鼠,随机分为5组:假手术组、对照组、超短波组、BMSCs组、超短波+BMSCs组,采用改良Allen′s法制作大鼠SCI模型,术后1 d、1周、2周、3周、4周用BBB评分法评价后肢运动功能的恢复情况。术后4周取材,行GFAP和ED-1免疫组化染色,测定其蛋白表达的积分光密度值,进行比较分析。结果术后4周超短波组、超短波+BMSCs组的BBB评分显著高于对照组,差异有统计学意义(均P<0.001),BMSCs组与对照组比较无统计学差异。仅超短波+BMSCs组GFAP的阳性表达显著低于对照组,差异有统计学意义(P<0.05)。超短波组、BMSCs组、超短波+BMSCs组ED-1表达水平明显低于对照组,差异有统计学意义(均P<0.05)。结论单纯超短波治疗即可明显改善SCI后神经功能恢复;超短波及BMSCs移植治疗均可减轻炎症,以超短波作用最为显著,二者联合治疗在促进功能恢复上并未显现出协同作用,但是在减轻炎性反应和胶质瘢痕形成上有协同作用。Objective To investigate the effects of bone marrow stromal cells（BMSCs）transplantation combined with low dose ultrashort wave（USW）radiation on functional recovery and the expression of glial fibrillary acidic protein（GFAP）and ED-1 after spinal cord injury（SCI）in rats,and further discuss its action mechanism. Methods Female Sprague-Dawley rats（n = 30）were randomly divided into 5 groups：sham-operated,as well as control,USW,BMSCs,and USW＋BMSCs that were subjected to spinal cord injury（SCI）. Basso-Beattie-Bresnahan（BBB）tests were carried out before the operation and at 1 d,1 week,2 weeks,3 weeks,4 weeks after SCI. 4 weeks later,animals were sacrificed and tissues were collected to make paraffin section. Immunohistochemical staining was performed to observe the expression of GFAP and ED-1. Results 4weeks after SCI,BBB scores were significantly higher in the USW and USW＋BMSCs groups than in the control group（both P〈0.001）. No significant difference was observed between the BMSCs group and control group. On the expression of GFAP,only USW＋BMSCs group showed significantly decreased compared with the control group（P〈0.05）. All treatment groups exhibited lower ED-1 expression than the control group（all P〈0.05）. Conclusion Our results indicate that USW radiation alone can obviously improve neural functional recovery after SCI. The USW radiation and BMSCs transplantation treatment can reduce inflammation,and USW radiation is more effective. The combination therapy did not show a synergistic action on promoting functional recovery,but do have an effect on reducing the inflammatory response and glial scar formation.

关 键 词：脊髓损伤 骨髓间充质干细胞 超短波 胶质纤维酸性蛋白 ED-1 

分 类 号：R651.2[医药卫生—外科学]