ω-6大豆油脂肪乳对小鼠腹泻的保护作用  

Protective Effect of ω-6 Soybean Oil Fat Emulsion on Folium Sennae-induced Diarrhea in Mice

作　　者：孙明立[1] 赵海山[1] 姚维范[1] 魏敏杰[1] 

机构地区：[1]中国医科大学药学院药理教研室,沈阳110122

出　　处：《中国医科大学学报》2016年第8期742-745,共4页Journal of China Medical University

摘　　要：目的探讨ω-6大豆油脂肪乳对番泻叶导致的小鼠腹泻的保护作用。方法将36只昆明小鼠随机分为对照组、腹泻组和ω-6大豆油脂肪乳组,每组12只。利用番泻叶制备小鼠腹泻模型。造模后,ω-6大豆油脂肪乳组注射ω-6大豆油脂肪乳(15m L/kg,每日1次),对照组与腹泻组小鼠每日尾静脉注射等剂量生理盐水,连续给药10 d。自造模之日起动态观察小鼠一般状态并记录小鼠体质量;并于给药前及给药第1、4、7和10天分别计算小鼠腹泻指数;给药10 d后处死小鼠,采用HE染色法观察小鼠小肠黏膜形态学变化;采用生化法检测小鼠血浆白蛋白(ALB)水平;采用免疫组化检测小鼠小肠黏膜组织中增殖细胞核抗原(PCNA)的表达。结果与腹泻组相比,ω-6大豆油脂肪乳组小鼠的一般状况和体质量明显改善,小鼠腹泻指数明显降低;给药10 d后,与腹泻组相比,ω-6大豆油脂肪乳组小鼠小肠黏膜损伤明显改善、血浆ALB水平显著升高,小肠黏膜中PCNA表达显著升高(P<0.05)。结论ω-6大豆油脂肪乳对番泻叶所致的小鼠腹泻具有显著的保护作用,其机制可能与上调PCNA表达水平有关。Objective To investigate the protective effect of ω-6 soybean oil fat emulsion on folium sennae-induced diarrhea in mice. Methods Thirty-six Kunming mice were randomly divided into three groups,including control,diarrhea,ω-6 soybean oil fat emulsion group（12 mice in each group）. Besides the mice in control group,other mice were administrated folium sennae by gavage for 15 days to establish the diarrhea model.Then mice in ω-6 soybean oil fat emulsion group received ω-6 soybean oil fat emulsion by intravenously administration at a dose of 15 m L/kg daily since 6th day after intragastric administration of folium sennae for 10 days. Animals in control group and diarrhea group were intravenously administered with same volume of saline. The body weight,general state and diarrhea index of the mice in each group were dynamically assessed. Ten days after intravenous injection,mice in every group were sacrificed and tissues were collected. Morphology of intestine mucosa was observed after HE staining. Albumin（ALB）level in plasma was evaluated by biochemical method. Proliferating cell nuclear antigen（PCNA）expression in intestine mucosa were assessed by immunohistochemical method. Results Compared with that in the diarrhea group,the general status,body weight and diarrhea index of mice in ω-6 soybean oil fat emulsion group were improved significantly. Ten days after intravenously administration,pathological change in intestine mucosa of mice in ω-6 soybean oil fat emulsion group was improved significantly,ALB level in plasma and PCNA expression in intestine mucosa were significantly increased（P〈0.05）compared with that in diarrhea group. Conclusion ω-6 soybean oil fat emulsion has a significant protective effect on the diarrhea of mice induced by folium sennae,which may be related to the up-regulated expression of PCNA by ω-6soybean oil fat emulsion.

关 键 词：ω-6大豆油脂肪乳 番泻叶 腹泻 小肠黏膜损伤 

分 类 号：R965.1[医药卫生—药理学]