微孢子虫对二点委夜蛾致病力研究  

Control Efficacy of One Microsporidian against Athetis lepigone Larvae

作　　者：张海剑[1] 宋健[1] 杜立新[1] 杨云鹤[1] 石洁[1] 

机构地区：[1]河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业部华北北部作物有害生物综合治理重点实验室,保定071000

出　　处：《中国生物防治学报》2016年第4期462-467,共6页Chinese Journal of Biological Control

基　　金：公益性行业(农业)科研专项(201303026);国家现代农业产业技术体系(CARS-02);河北省农林科学院科学技术研究与发展计划项目(A2015120302)

摘　　要：本研究对分离自二点委夜蛾Athetis lepigone(M?schler)越冬幼虫体内的微孢子虫进行形态学和分子生物学鉴定,初步证明该微孢子虫与家蚕微孢子虫具有最近的近缘关系,属于微孢子纲,微孢子虫目,微粒子科,微粒子属Nosema;测定了该微孢子虫不同浓度对不同龄期二点委夜蛾的致病力,在接种1×107孢子/m L浓度时,对初孵、2龄、3龄幼虫8 d的校正死亡率分别达到91.6%、83.35%和70.4%,显示出对靶标害虫具有较好的毒力;对田间自然条件下微孢子虫在二点委夜蛾种群中的流行动态进行了调查评估,结果表明微孢子虫在二点委夜蛾种群数量的控制中起到一定作用,具有潜在的应用价值。A microsporidian was isolated from Athetis lepigone overwintering larvae, based on morphological and molecular biology experiments, it was preliminarily proved that the rnicrosporidian had the closest relationship with Nosema bombycis, which belong to Microsporea, Microspoida, Nosematida, Nosema. Bioassay tests showed that microsporidian had high toxicity against different instars larvae of A. lepigone. The corrected mortality of the concentration I × 107 spores/mL to initial hatching larva, 2nd instar larva and 3rd instar larva at 8 d after infection were 91.6%, 83.35% and 70.4%. In field investigation, we concluded that the microsporidian could controll A. lepigone populations with potential application value.

关 键 词：二点委夜蛾 微孢子虫 致病力 流行动态 

分 类 号：S476[农业科学—农业昆虫与害虫防治]